Niwa M, Hara A, Iwai T, Wang S, Hotta K, Mori H, Uematsu T
Department of Pharmacology, Gifu University School of Medicine, Japan.
Neurosci Lett. 2001 Mar 9;300(2):103-6. doi: 10.1016/s0304-3940(01)01559-2.
To determine whether apoptotic process is involved in the delayed neuronal death in hippocampal CA1 region following forebrain ischemia in gerbils, time dependent activation of caspase and DNA fragmentation were evaluated by immuno-staining and terminal dUTP nick-end-labeling staining, respectively. After transient forebrain ischemia in gerbils, activation of apoptosis related caspase, including caspase-3, was apparent, and it preceded DNA fragmentation in CA1 region. These observations suggest that apoptotic process is involved in hippocampal delayed neuronal death.
为了确定凋亡过程是否参与沙鼠前脑缺血后海马CA1区的迟发性神经元死亡，分别通过免疫染色和末端脱氧核苷酸转移酶介导的缺口末端标记染色评估了半胱天冬酶的时间依赖性激活和DNA片段化。在沙鼠短暂前脑缺血后，包括半胱天冬酶-3在内的凋亡相关半胱天冬酶的激活很明显，并且在CA1区的DNA片段化之前出现。这些观察结果表明凋亡过程参与了海马迟发性神经元死亡。
