轻度低温减轻短暂性全脑缺血后沙土鼠CA1锥体神经元顶树突中DNA片段转运增加的现象

Increase of fragmented DNA transport in apical dendrites of gerbil CA1 pyramidal neurons following transient forebrain ischemia by mild hypothermia.

作者信息

Hara A, Niwa M, Iwai T, Yano H, Bunai Y, Uematsu T, Yoshimi N, Mori H

机构信息

Department of Pathology, Gifu University School of Medicine, Japan.

出版信息

Neurosci Lett. 2000 Feb 11;280(1):73-7. doi: 10.1016/s0304-3940(99)00979-9.

DOI:10.1016/s0304-3940(99)00979-9

PMID:10696815

Abstract

Mild hypothermia (38 degrees C) accelerated transport of fragmented DNA in apical dendrites of the gerbil CA1 pyramidal neurons and increased dendrite-terminal fragmented DNA pooling in the apoptotic process following transient forebrain ischemia. The specific DNA fragmentation after the ischemic insult in gerbil hippocampus was examined by in situ nick-end-labeling method, and fluorescence DNA detection technique by DAPI was also performed. There is a precise temperature dependence for the migration of fragmented DNA from nuclei into apical dendrites of CA1 pyramidal cells during apoptosis following transient forebrain ischemia. Increase of fragmented DNA pooling is highly temperature sensitive, occurring at 38 degrees C, while at 39 degrees C there is a marked decrease in DNA pooling.

摘要

轻度低温（38摄氏度）加速了沙土鼠CA1锥体神经元顶树突中碎片化DNA的转运，并在短暂性前脑缺血后的凋亡过程中增加了树突末端碎片化DNA的聚集。采用原位缺口末端标记法检测沙土鼠海马缺血损伤后的特异性DNA片段化，并采用DAPI荧光DNA检测技术进行检测。在短暂性前脑缺血后的凋亡过程中，碎片化DNA从细胞核迁移到CA1锥体细胞顶树突的过程存在精确的温度依赖性。碎片化DNA聚集的增加对温度高度敏感，发生在38摄氏度时，而在39摄氏度时DNA聚集明显减少。