Population data for the STR systems D8S1132, CD4, VWA and TH01 in the region of Podlasie (northeastern Poland).
作者信息
Pepiński W, Niemcunowicz-Janica A, Janica J, Skawrońska M, Koc-Zórawska E, Rydzewska M, Sołtyszewski I
机构信息
Department of Forensic Medicine, Medical University, Białystok, Poland.
出版信息
Med Sci Monit. 2001 Jan-Feb;7(1):130-3.
BACKGROUND
STR systems are widely used in forensic casework due to their discriminating power and high sensitivity. The aim of the study was to determine allele frequencies and forensic efficiency parameters of the STRs D8S1132, CD4, VWA and TH01 in a population sample of Podlasie (Northeastern Poland).
MATERIAL AND METHODS
Blood specimens were collected from the unrelated individuals in Podlasie, Poland. The DNA samples were extracted with Chelex method and amplified by PCR technique. The PCR products were analyzed by the PAG horizontal electrophoresis with discontinuous buffer system and then silver stained.
RESULTS
All common alleles included in the allelic ladders were found. For all loci examined no deviations from Hardy-Weinberg equilibrium were found.
CONCLUSION
The TH01, VWA and D8S1132 systems have been confirmed as particularly useful in the paternity testing and the individualization of biological evidence.
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