波兰东北部波德拉谢地区STR系统D8S1132、CD4、VWA和TH01的群体数据。
Population data for the STR systems D8S1132, CD4, VWA and TH01 in the region of Podlasie (northeastern Poland).
作者信息
Pepiński W, Niemcunowicz-Janica A, Janica J, Skawrońska M, Koc-Zórawska E, Rydzewska M, Sołtyszewski I
机构信息
Department of Forensic Medicine, Medical University, Białystok, Poland.
出版信息
Med Sci Monit. 2001 Jan-Feb;7(1):130-3.
BACKGROUND
STR systems are widely used in forensic casework due to their discriminating power and high sensitivity. The aim of the study was to determine allele frequencies and forensic efficiency parameters of the STRs D8S1132, CD4, VWA and TH01 in a population sample of Podlasie (Northeastern Poland).
MATERIAL AND METHODS
Blood specimens were collected from the unrelated individuals in Podlasie, Poland. The DNA samples were extracted with Chelex method and amplified by PCR technique. The PCR products were analyzed by the PAG horizontal electrophoresis with discontinuous buffer system and then silver stained.
RESULTS
All common alleles included in the allelic ladders were found. For all loci examined no deviations from Hardy-Weinberg equilibrium were found.
CONCLUSION
The TH01, VWA and D8S1132 systems have been confirmed as particularly useful in the paternity testing and the individualization of biological evidence.
背景
由于其鉴别能力和高灵敏度，STR系统广泛应用于法医案件检测。本研究的目的是确定在波兰东北部波德拉谢省的一个人群样本中STR基因座D8S1132、CD4、VWA和TH01的等位基因频率和法医效率参数。
材料与方法
从波兰波德拉谢省的无关个体中采集血液样本。采用Chelex法提取DNA样本，并通过PCR技术进行扩增。PCR产物采用不连续缓冲系统的PAG水平电泳进行分析，然后进行银染。
结果
发现了等位基因阶梯中包含的所有常见等位基因。在所检测的所有基因座中，均未发现偏离哈迪-温伯格平衡的情况。
结论
TH01、VWA和D8S1132系统已被证实在亲子鉴定和生物证据个体化方面特别有用。
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