大肠杆菌中umuDC与dnaQ的相互作用及其对细胞周期调控和SOS诱变的影响
umuDC-dnaQ Interaction and its implications for cell cycle regulation and SOS mutagenesis in Escherichia coli.
作者信息
Sutton M D, Murli S, Opperman T, Klein C, Walker G C
机构信息
Biology Department, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
出版信息
J Bacteriol. 2001 Feb;183(3):1085-9. doi: 10.1128/JB.183.3.1085-1089.2001.
Abstract
The Escherichia coli SOS-regulated umuDC gene products participate in a DNA damage checkpoint control and in translesion DNA synthesis. Specific interactions involving the UmuD and UmuD' proteins, both encoded by the umuD gene, and components of the replicative DNA polymerase, Pol III, appear to be important for regulating these two biological activities of the umuDC gene products. Here we show that overproduction of the epsilon proofreading subunit of Pol III suppresses the cold sensitivity normally associated with overexpression of the umuDC gene products. Our results suggest that this suppression is attributable to specific interactions between UmuD or UmuD' and the C-terminal domain of epsilon.
摘要
大肠杆菌SOS调节的umuDC基因产物参与DNA损伤检查点控制和跨损伤DNA合成。由umuD基因编码的UmuD和UmuD'蛋白与复制性DNA聚合酶Pol III的组分之间的特异性相互作用，似乎对调节umuDC基因产物的这两种生物学活性很重要。在此我们表明，Pol III的ε校对亚基的过量表达抑制了通常与umuDC基因产物过表达相关的冷敏感性。我们的结果表明，这种抑制归因于UmuD或UmuD'与ε的C末端结构域之间的特异性相互作用。
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The SOS response: recent insights into umuDC-dependent mutagenesis and DNA damage tolerance.SOS 应答：关于 UmuDC 依赖性诱变和 DNA 损伤耐受性的最新见解
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