大肠杆菌DinB（DNA聚合酶IV）的人类和小鼠同源物，属于UmuC/DinB超家族成员。
Human and mouse homologs of Escherichia coli DinB (DNA polymerase IV), members of the UmuC/DinB superfamily.
作者信息
Gerlach V L, Aravind L, Gotway G, Schultz R A, Koonin E V, Friedberg E C
机构信息
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75235-9072, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11922-7. doi: 10.1073/pnas.96.21.11922.
Abstract
To understand the mechanisms underlying mutagenesis in eukaryotes better, we have cloned mouse and human homologs of the Escherichia coli dinB gene. E. coli dinB encodes DNA polymerase IV and greatly increases spontaneous mutations when overexpressed. The mouse and human DinB1 amino acid sequences share significant identity with E. coli DinB, including distinct motifs implicated in catalysis, suggesting conservation of the polymerase function. These proteins are members of a large superfamily of DNA damage-bypass replication proteins, including the E. coli proteins UmuC and DinB and the Saccharomyces cerevisiae proteins Rev1 and Rad30. In a phylogenetic tree, the mouse and human DinB1 proteins specifically group with E. coli DinB, suggesting a mitochondrial origin for these genes. The human DINB1 gene is localized to chromosome 5q13 and is widely expressed.
摘要
为了更好地理解真核生物中诱变作用的潜在机制，我们克隆了大肠杆菌dinB基因的小鼠和人类同源基因。大肠杆菌dinB编码DNA聚合酶IV，过表达时会显著增加自发突变。小鼠和人类的DinB1氨基酸序列与大肠杆菌DinB具有显著的同源性，包括与催化作用相关的独特基序，这表明聚合酶功能具有保守性。这些蛋白质是DNA损伤旁路复制蛋白的一个大型超家族的成员，包括大肠杆菌的UmuC和DinB蛋白以及酿酒酵母的Rev1和Rad30蛋白。在系统发育树中，小鼠和人类的DinB1蛋白与大肠杆菌DinB特异性地归为一组，表明这些基因起源于线粒体。人类DINB1基因定位于染色体5q13，并且广泛表达。
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本文引用的文献
1
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Mol Cell. 1999 Aug;4(2):281-6. doi: 10.1016/s1097-2765(00)80376-7.
2
Novel DNA polymerases offer clues to the molecular basis of mutagenesis.新型DNA聚合酶为诱变的分子基础提供了线索。
Cell. 1999 Aug 20;98(4):413-6. doi: 10.1016/s0092-8674(00)81970-4.
3
hRAD30 mutations in the variant form of xeroderma pigmentosum.着色性干皮病变异型中的hRAD30基因突变。
Science. 1999 Jul 9;285(5425):263-5. doi: 10.1126/science.285.5425.263.
4
The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta.着色性干皮病变异型（XPV）基因编码人类DNA聚合酶η。
Nature. 1999 Jun 17;399(6737):700-4. doi: 10.1038/21447.
5
Xeroderma pigmentosum variant (XP-V) correcting protein from HeLa cells has a thymine dimer bypass DNA polymerase activity.来自人宫颈癌细胞系的着色性干皮病变异型（XP-V）校正蛋白具有胸腺嘧啶二聚体旁路DNA聚合酶活性。
EMBO J. 1999 Jun 15;18(12):3491-501. doi: 10.1093/emboj/18.12.3491.
6
Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Poleta.酵母DNA聚合酶Poleta对胸腺嘧啶-胸腺嘧啶二聚体的高效旁路作用。
Science. 1999 Feb 12;283(5404):1001-4. doi: 10.1126/science.283.5404.1001.
7
Conserved domains in DNA repair proteins and evolution of repair systems.DNA修复蛋白中的保守结构域与修复系统的进化
Nucleic Acids Res. 1999 Mar 1;27(5):1223-42. doi: 10.1093/nar/27.5.1223.
8
Iterated profile searches with PSI-BLAST--a tool for discovery in protein databases.使用PSI-BLAST进行迭代序列谱搜索——一种在蛋白质数据库中进行发现的工具。
Trends Biochem Sci. 1998 Nov;23(11):444-7. doi: 10.1016/s0968-0004(98)01298-5.
9
Role of the first aspartate residue of the "YxDTDS" motif of phi29 DNA polymerase as a metal ligand during both TP-primed and DNA-primed DNA synthesis.φ29 DNA聚合酶“YxDTDS”基序的首个天冬氨酸残基在TP引发和DNA引发的DNA合成过程中作为金属配体的作用。
J Mol Biol. 1998 Oct 30;283(3):633-42. doi: 10.1006/jmbi.1998.2121.
10
The mutagenesis proteins UmuD' and UmuC prevent lethal frameshifts while increasing base substitution mutations.诱变蛋白UmuD'和UmuC可防止致死性移码，同时增加碱基置换突变。
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