Hong So Gun, Kim Min Kyu, Jang Goo, Oh Hyun Ju, Park Jung Eun, Kang Jung Taek, Koo Ok Jae, Kim Teoan, Kwon Mo Sun, Koo Bon Chul, Ra Jeong Chan, Kim Dae Yong, Ko CheMyong, Lee Byeong Chun
Seoul National University, Korea.
Genesis. 2009 May;47(5):314-22. doi: 10.1002/dvg.20504.
Dogs (Canis familiaris) share many common genetic diseases with humans and development of disease models using a transgenic approach has long been awaited. However, due to the technical difficulty in obtaining fertilizable eggs and the unavailability of embryonic stem cells, no transgenic dog has been generated. Canine fetal fibroblasts were stably transfected with a red fluorescent protein (RFP) gene-expressing construct using retrovirus gene delivery method. Somatic cell nuclear transfer was then employed to replace the nucleus of an oocyte with the nucleus of the RFP-fibroblasts. Using this approach, we produced the first generation of transgenic dogs with four female and two male expressing RFP.
狗（犬科动物）与人类有许多共同的遗传疾病，人们长期以来一直期待利用转基因方法开发疾病模型。然而，由于获取可受精卵子存在技术困难以及胚胎干细胞不可用，尚未培育出转基因狗。使用逆转录病毒基因递送方法，将表达红色荧光蛋白（RFP）基因的构建体稳定转染犬胎儿成纤维细胞。然后采用体细胞核移植，用RFP成纤维细胞的细胞核替换卵母细胞的细胞核。通过这种方法，我们培育出了第一代转基因狗，其中四只雌性和两只雄性表达RFP。
