Wei G H, Chen W X, Zhu M E
Department of Resource and Environment Science, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China.
Yi Chuan Xue Bao. 2000;27(11):1027-32.
Based on the previous studies on numerical taxonomy and SDS-PAGE of whole-cell protein, the rhizobia strains isolated from Indigofera sp. in loess plateau area of North-west China constituted a new cluster, the 16S rDNA sequence of representative strain SHL042 was tested, and the phylogenetic tree was produced. In this tree, the strain SHL042, R. tropici A, R. tropici B, R. leguminosarum, R. etli, R. hananesis, R. mongolense and R. gallicum constituted a branch of phylogenetic. Within this branch, the similarity values of 16S rDNA sequences were 95.4%, 95.5%, 96.3%, 95.8%, 96.3%, 97.9% and 97.7% respectively. The values were more than 95%. This indicated that these species should belong to the same genus. The values of DNA homology in the new cluster were all more than 80%, but the values between SHL042 and the strains of these species were less than 50%. Thus, the strain SH714 represented a new rhizobia species.
基于先前关于数值分类学和全细胞蛋白SDS - PAGE的研究，从中国西北黄土高原地区的槐蓝属植物中分离出的根瘤菌菌株构成了一个新的类群，对代表性菌株SHL042的16S rDNA序列进行了检测，并构建了系统发育树。在这棵树中，菌株SHL042、热带根瘤菌A、热带根瘤菌B、豌豆根瘤菌、埃氏根瘤菌、哈氏根瘤菌、蒙古根瘤菌和加氏根瘤菌构成了一个系统发育分支。在这个分支内，16S rDNA序列的相似性值分别为95.4%、95.5%、96.3%、95.8%、96.3%、97.9%和97.7%。这些值均超过95%。这表明这些物种应属于同一属。新类群中的DNA同源性值均超过80%，但SHL042与这些物种的菌株之间的值小于50%。因此，菌株SH714代表了一种新的根瘤菌物种。
