Yan A, Chen W
China Agriculture University, Biological College, Beijing 100094.
Wei Sheng Wu Xue Bao. 2000 Feb;40(1):1-8.
On the basis of numerical taxonomy, SDS-PAGE whole-cell protein analysis, 16S rDNA PCR-RFLP and DNA-DNA hybridization, the full length of 16SrDNA of two strains (XJ96060, XJ96408) were sequenced and compared with the 16S rDNA sequences of other members of the alpha 2 subclass of the Proteobacteria available from GenBank. An unrooted tree was produced to determine the phylogenetic relationships of two groups. The results showed that all strains were clustered into 6 branches, they were Sinorhizobium, Mesorhizobium, Agrobacterium-Rhizobium, Bradyrhizobium. Rhizobium, Azorhizobium. Group 1, 2 fell into Sinorhizobium branch.
基于数值分类法、SDS - PAGE全细胞蛋白分析、16S rDNA PCR - RFLP和DNA - DNA杂交技术，对两株菌（XJ96060、XJ96408）的16S rDNA全长进行测序，并与GenBank中变形菌门α2亚类其他成员的16S rDNA序列进行比较。构建了一棵无根树以确定两组菌的系统发育关系。结果表明，所有菌株聚为6个分支，分别为中华根瘤菌属、中慢生根瘤菌属、土壤杆菌 - 根瘤菌属、慢生根瘤菌属、根瘤菌属、固氮根瘤菌属。第1、2组归入中华根瘤菌属分支。
