Yuan Y P, Chen X, Xiao S H, Xin Z Y, Zhang Z Y, Lin Z S, Ma Y Z, Hu H Q
Key Laboratory of Agricultural Ministry for Crop Genetics and Breeding, Institute of Crop Breeding and Cultivation, CAAS, Beijing 100081, China.
Yi Chuan Xue Bao. 2000;27(12):1080-3.
The barley chromosome in wheat was identified by genomic in situ hybridization (GISH) in which biotin labelled total genomic DNA of barley Betzes was used as probe and the unlabelled total DNA of common wheat Chinese Spring (CS) as blocking DNA. A series of wheat materials were tested as follows: two disomic alien substitution and monosomic alien addition lines, 2n = 43; two monosomic alien substitution lines, 2n = 42; seven disomic alien substitution lines, 2n = 42. RFLP probe psr131 on the short arm of the homologous group 2 was used to analyze the barley chromosome in wheat. The result indicated that there was a same band in barley Betzes and substitution line A5. The chromosome 2A of A5 was substituted by the chromosome 2H of barley. These materials will be useful in transferring the valuable genes in the chromosome 2H to wheat.
通过基因组原位杂交（GISH）鉴定了小麦中的大麦染色体，其中以生物素标记的大麦Betzes总基因组DNA作为探针，以未标记的普通小麦中国春（CS）总DNA作为封阻DNA。对一系列小麦材料进行了如下测试：两个二体异代换系和单体异附加系，2n = 43；两个单体异代换系，2n = 42；七个二体异代换系，2n = 42。使用同源群2短臂上的RFLP探针psr131分析小麦中的大麦染色体。结果表明，大麦Betzes和代换系A5中有一条相同的带。A5的2A染色体被大麦的2H染色体所取代。这些材料将有助于将2H染色体上的有价值基因转移到小麦中。
