Liu G Q, Jiao C Z, Jiang R Q, Zhao S M, Xu J X, Zhang X X, Liang Z L
Institute of Genetics, Chinese Academy of Scieucos, Beijing 100101, China.
Yi Chuan Xue Bao. 2000;27(12):1094-9.
To study the effectiveness of Gossypium interspecific hybrid breeding on molecular level, the six germplasm lines from interspecific hybrids between G. hirsutum and G. bickii were analysed by the techniques of RAPD and isozyme electrophoresis. The main results were summarized as follows: (1) The basic zymograms of peroxidase isozyme of the six germplasm lines were similar to their G. hirsutum parent, but two out of the germplasm lines showed one specific band of the wild parent of G. bickii (pI 4.85); (2) The RAPD fingerprint showed distinct difference of genomic DNA among the germplasm lines; (3) The four specific DNA fragments of G. bickii were observed in RAPD patters of four out of the six germplasm lines.
为了在分子水平上研究陆地棉种间杂交育种的效果，利用RAPD技术和同工酶电泳技术对陆地棉与比克氏棉种间杂种的6个种质系进行了分析。主要结果如下：（1）6个种质系过氧化物酶同工酶的基本酶谱与其陆地棉亲本相似，但其中2个种质系出现了比克氏棉野生亲本的一条特异性带（pI 4.85）；（2）RAPD指纹图谱显示种质系间基因组DNA存在明显差异；（3）在6个种质系中的4个种质系的RAPD图谱中观察到了比克氏棉的4个特异性DNA片段。
