Liu G Q, Jiao C Z, Jiang R Q, Zhang X X, Jiang B G, Zhao S M, Xu J X, Liang Z L
Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.
Yi Chuan Xue Bao. 2000;27(11):999-1005.
To study the genetic character of trispecific hybrid cultivar Shiyuan 321, we analyzed this cultivar and its parents by isozyme and RAPD techniques. The main results are as follows: (1) Esterase isozyme analysis showed that there was one specific band among cultivar Suiyuan 321 and its parents, G. thurberi and G. barbadense; (2) Six specific DNA fragments amplified by 4 primers were found among cultivar Shiyuan 321 and its parents G. barbadense, G. thurberi; (3) By comparing RAPD differences among Shiyuan 321 and its parents, it is indicated that the genetic heterogeneity of cultivar Shiyuan 321 is very high at its genomic DNA level. The above results proved that Shiyuan 321 is a trispecific cultivar from G. barbadense x G. thurberi x G. hirsutum as well as a new germplasm.
为研究三交种棉花品种石远321的遗传特性，我们采用同工酶和RAPD技术对该品种及其亲本进行了分析。主要结果如下：（1）酯酶同工酶分析表明，在品种石远321及其亲本瑟伯氏棉和海岛棉中存在一条特异性条带；（2）在品种石远321及其亲本海岛棉、瑟伯氏棉中发现了由4条引物扩增出的6个特异性DNA片段；（3）通过比较石远321与其亲本之间的RAPD差异，表明石远321品种在基因组DNA水平上的遗传异质性非常高。上述结果证明，石远321是由海岛棉×瑟伯氏棉×陆地棉育成的三交种品种，也是一份新种质。
