Chen J F, Ying J, Wang S L, Liu Z H, Qi L L, Chen P D
Key Laboratory of Crop Cytogenetics, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan Xue Bao. 2001;28(1):52-5.
Chinese Spring phlb mutant (C S phlbphlb) was crossed to Triticum aestivum-Haynaldia villosa 6V (6A) alien substitution line and F1 back was crossed with C. S phlbphlb. One LV 02 with varied H. villosa 6V chromosome and one LV 02-01 with 40 T. aestivum chromosome, one H. villosa 6V and 6VS chromosome were screened in BC1F1 and BC1F2 respectively by C-banding and the fluorescence in situ hybridization (FISH). In segregated generation of LV 02-01, eight T. aestivum-H. villosa 6VS ditelosomic substitution lines were screened by FISH and C-banding.
中国春phlb突变体（C S phlbphlb）与普通小麦-簇毛麦6V（6A）异代换系杂交，F1代回交C S phlbphlb。分别通过C分带和荧光原位杂交（FISH）技术，在BC1F1和BC1F2中筛选出1个具有不同簇毛麦6V染色体的LV 02、1个具有40条普通小麦染色体、1条簇毛麦6V和6VS染色体的LV 02-01。在LV 02-01的分离世代中，通过FISH和C分带筛选出8个普通小麦-簇毛麦6VS双端体代换系。
