Chen Quan-Zhan, Qi Zeng-Jun, Feng Yi-Gao, Wang Su-Ling, Chen Pei-Du
Key Laboratory of Crop Cytogenetics, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan Xue Bao. 2002 Apr;29(4):355-8.
Chromosome 3C of Aegilops triuncialis was discovered with ability to be transferred preferentially in the case of its monosomic status in wheat background, whereas, those gametes without 3C would result in chromosome structural changes including deletions and translocations. In the present study, Triticum aestivum-Haynaldia villosa substitution line 4V(4D) developed in our laboratory, was crossed to T. aestivum c.v. Norin 26-Aegilops triuncialis 3C addition line, and the hybrids F1 were then backcrossed with common wheat in order to induce structural changes of 4V. Both chromosome C-banding and genomic in situ hybridization was applied to search such chromosome variations. In this case, total genomic DNA of Haynaldia villosa was labelled by Biotin-11-dUTP as probes and total genomic DNA of Chinese Spring as the block. Moreover, several chromosome changes within common wheat such as isochromosome 1BL.1BL(B39-2) and others were also revealed. The result indicated that two translocation lines T4VL.3AS(A47-10-3) and T4VS.4DL(A47-25-4), two telocentric chromosome lines A47-7-2(4VS) and A47-32-2(4VL), and two isochromosomes including 4VS.4VS(A47-23) and 4VL.4VL(A412-5-4) were identified from BC1F2 or BC1F3. This result indicated that gametocidal chromosome 3C of Aegilop triuncialis could effectively induce structural changes of both chromosome 4V of Haynaldia villosa and chromosomes of wheat.
在小麦背景下，发现节节麦的3C染色体在单体状态时具有优先转移的能力，而不含3C的配子会导致包括缺失和易位在内的染色体结构变化。在本研究中，将本实验室培育的普通小麦-绒毛草4V(4D)代换系与普通小麦品种Norin 26-节节麦3C附加系杂交，然后将杂种F1与普通小麦回交，以诱导4V染色体的结构变化。采用染色体C带和基因组原位杂交技术来寻找这种染色体变异。在这种情况下，用生物素-11-dUTP标记绒毛草的总基因组DNA作为探针，以中国春的总基因组DNA作为封阻。此外，还揭示了普通小麦中的一些染色体变化，如1BL.1BL等臂染色体(B39-2)。结果表明，从BC1F2或BC1F3中鉴定出两个易位系T4VL.3AS(A47-10-3)和T4VS.4DL(A47-25-4)、两个端着丝粒染色体系A47-7-2(4VS)和A47-32-2(4VL)，以及两个等臂染色体4VS.4VS(A47-23)和4VL.4VL(A412-5-4)。这一结果表明，节节麦的杀配子染色体3C能有效地诱导绒毛草4V染色体和小麦染色体的结构变化。
