Sion-Vardy N, Fliss D M, Prinsloo I, Shoham-Vardi I, Benharroch D
Institute of Pathology, Soroka University Medical Center, Beer Sheva, Israel.
Pathol Res Pract. 2001;197(1):1-5. doi: 10.1078/0344-0338-00001.
Tumor angiogenesis has been related to tumor growth and an increased probability of metastatic spread. Previous studies have led to conflicting views regarding the prognostic significance of angiogenesis in squamous cell carcinoma of the head and neck. To evaluate the role of tumor angiogenesis in the biology of squamous cell carcinoma of the larynx, we quantified the microvascular network in 59 primary laryngeal carcinomas and looked for an association with outcome. Microvessels were stained immunohistochemically using antibodies for factor VIII-related antigen and the antibody JC70 (CD-31). In each case, microvessels were counted in three fields at x200 magnification, in areas of most intense neovascularization. We found a significantly higher number of microvessels in tumors showing deeper levels of invasion.
肿瘤血管生成与肿瘤生长以及转移扩散概率的增加有关。先前的研究对于头颈部鳞状细胞癌中血管生成的预后意义产生了相互矛盾的观点。为了评估肿瘤血管生成在喉鳞状细胞癌生物学中的作用，我们对59例原发性喉癌中的微血管网络进行了定量分析，并寻找其与预后的关联。使用针对因子VIII相关抗原的抗体和JC70抗体（CD-31）通过免疫组织化学方法对微血管进行染色。在每例病例中，在x200放大倍数下，于新生血管最密集的区域的三个视野中对微血管进行计数。我们发现，在显示出更深浸润程度的肿瘤中，微血管数量显著更多。
