Laitakari J, Näyhä V, Stenbäck F
Department of Pathology, University of Oulu, PO Box 5000, Oulu 90014, Finland.
J Clin Pathol. 2004 Apr;57(4):394-401. doi: 10.1136/jcp.2002.004978.
Angiogenesis and vessel organisation in laryngeal tumour development and progression were examined to determine characteristics of biological and clinical relevance.
Automated quantitative image analysis was performed on 1451 factor VIII (FVIII) associated blood vessels with regard to occurrence, structure, size, shape, and staining intensity, in addition to vessel direction.
Vessel numbers were increased in preneoplastic states and severe dysplasia, in addition to squamous cell carcinomas, being greater in poorly differentiated carcinomas. Small regular vessels predominated in benign conditions and large, irregular vessels in malignant neoplasms. Vessel distribution was related to degree of differentiation in squamous cell carcinomas, with circumferential angiogenesis occurring in well differentiated neoplasms, directional angiogenesis in moderately differentiated tumours, and aberrant angiogenesis in less well differentiated neoplasms. Alterations in vessel shape increased significantly with increasing degree of malignancy. Comparing the characteristics of individual vessels showed vessel shape abnormalities and the intensity of FVIII staining to increase with vessel size.
Increased angiogenesis was an early event in laryngeal tumour development, with vessel structure, size, and shape related to the tumour growth pattern and behaviour.
研究喉肿瘤发生发展过程中的血管生成和血管结构，以确定其生物学及临床相关性特征。
运用自动定量图像分析技术，对1451条与凝血因子VIII（FVIII）相关的血管的出现情况、结构、大小、形状、染色强度以及血管方向进行分析。
除鳞状细胞癌外，癌前状态和重度发育异常时血管数量增加，低分化癌中的血管数量更多。良性病变中以小的规则血管为主，恶性肿瘤中以大的不规则血管为主。鳞状细胞癌中的血管分布与分化程度有关，高分化肿瘤中出现周向血管生成，中分化肿瘤中出现定向血管生成，低分化肿瘤中出现异常血管生成。血管形状的改变随恶性程度增加而显著增加。比较单个血管的特征发现，血管形状异常和FVIII染色强度随血管大小增加而增加。
血管生成增加是喉肿瘤发生发展的早期事件，血管结构、大小和形状与肿瘤生长模式及行为相关。
