Kim S, Stair E L, Lish J W, Lochmiller R L, Rafferty D P, Qualls C W
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, USA.
J Toxicol Environ Health A. 2001 Jan 26;62(2):97-105. doi: 10.1080/009841001455517.
Various chemical mixtures exist in soil contaminated with petrochemical wastes, yet no comprehensive assessment of their impact on terrestrial ecosystems has been conducted. The purpose of this study was to evaluate hematotoxicity risks to wild populations of cotton rats (Sigmodon hispidus) residing in habitats previously contaminated by petroleum industrial wastes. Resident cotton rats were monitored on nine contaminated sites and nine ecologically matched reference sites in Oklahoma. The possible toxicological interactions of petrochemical wastes on bone marrow was investigated by using the assay of colony formation of granulocyte-macrophage progenitor cells. There was a consistent significant 21 to 39% decrease in the number of colony-forming units of granulocyte-macrophage (CFU-GM) in cotton rats from petrochemical-contaminated sites compared to matched reference sites, with no marked changes in hematological or histopathological parameters. These results suggest that bone-marrow progenitor cell culture is a sensitive indicator for the assessment of ecotoxicity risks associated with petrochemical wastes that are generated by the oil refining industry. Long-term exposure to hazardous wastes associated with the petroleum industry may represent a subtle risk to the hematopoietic system in humans.
受石化废物污染的土壤中存在各种化学混合物，但尚未对其对陆地生态系统的影响进行全面评估。本研究的目的是评估居住在先前受石油工业废物污染栖息地的野生棉鼠（Sigmodon hispidus）的血液毒性风险。在俄克拉荷马州的九个受污染地点和九个生态匹配的参考地点对当地的棉鼠进行了监测。通过粒细胞-巨噬细胞祖细胞集落形成试验，研究了石化废物对骨髓可能的毒理学相互作用。与匹配的参考地点相比，来自石化污染地点的棉鼠中粒细胞-巨噬细胞集落形成单位（CFU-GM）的数量持续显著减少21%至39%，血液学或组织病理学参数没有明显变化。这些结果表明，骨髓祖细胞培养是评估与炼油行业产生的石化废物相关的生态毒性风险的敏感指标。长期接触与石油工业相关的危险废物可能对人类造血系统构成潜在风险。
