Morita M, Wang H L
Department of Preventive Dentistry, Okayama University Dental School, Japan.
J Periodontol. 2001 Jan;72(1):74-8. doi: 10.1902/jop.2001.72.1.74.
Volatile sulfur compounds (VSC), such as hydrogen sulfide and methyl mercaptan, are toxic metabolites produced by periodontal pathogens. Their relationship to periodontal disease severity is not yet fully understood. Hence, the aims of this study were to: 1) examine the relationship between sulcular sulfide (pS) levels and severity of periodontal disease and 2) examine the link between pS level and the BANA (benzoyl-DL-arginine-naphthylamide) test.
Seventy systemically healthy subjects with a mean age of 53.0 +/- 13.8 years participated. Three sites were selected from each subject based upon radiographic bone loss (RBL): RBL < 2 mm, healthy; RBL > or = 2 to < 4, low to moderate; RBL > or = 4 mm, severe. Periodontal parameters, probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP), were recorded. The pS level was measured using a portable sulfide monitor in a digital score ranging from 0.0 (< 10(-7) M of S) to 5.0 (> or = 10(-2) M of S) in increments of 0.5. The presence of specific bacteria in subgingival plaque was detected using BANA test.
The mean pS level was 0.10 +/- 0.23, 0.36 +/- 0.48, and 1.10 +/- 0.87 for healthy, low to moderate, and severe disease sites, respectively, and was statistically different (P<0.001). The pS level was positively correlated with the BANA test, and was higher in untreated subjects than maintenance subjects (P<0.01).
The pS level may be a potential indicator for detecting severity of periodontal disease and identifying bacteria that are capable of hydrolyzing BANA.
挥发性硫化物（VSC），如硫化氢和甲硫醇，是牙周病原体产生的有毒代谢产物。它们与牙周疾病严重程度的关系尚未完全明确。因此，本研究的目的是：1）研究龈沟硫化物（pS）水平与牙周疾病严重程度之间的关系；2）研究pS水平与BANA（苯甲酰-DL-精氨酸萘酰胺）试验之间的联系。
70名全身健康的受试者参与研究，平均年龄为53.0±13.8岁。根据影像学骨丧失（RBL）情况从每位受试者中选取三个部位：RBL<2mm，健康；RBL≥2至<4mm，低至中度；RBL≥4mm，重度。记录牙周参数，包括探诊深度（PD）、临床附着水平（CAL）和探诊出血（BOP）。使用便携式硫化物监测仪测量pS水平，数字评分范围为0.0（<10⁻⁷M的S）至5.0（≥10⁻²M的S），增量为0.5。使用BANA试验检测龈下菌斑中特定细菌的存在。
健康、低至中度和重度疾病部位的平均pS水平分别为0.10±0.23、0.36±0.48和1.10±0.87，差异有统计学意义（P<0.001）。pS水平与BANA试验呈正相关，未经治疗的受试者的pS水平高于维持治疗的受试者（P<0.01）。
pS水平可能是检测牙周疾病严重程度和识别能够水解BANA的细菌的潜在指标。
