Nishiyama C, Takahashi K, Nishiyama M, Okumura K, Ra C, Ohtake Y, Yokota T
Foods & Pharmaceuticals Research & Development Laboratory, Asahi Breweries, Ltd., Kitasoma-gun, Ibaraki, Japan.
Biosci Biotechnol Biochem. 2000 Dec;64(12):2601-7. doi: 10.1271/bbb.64.2601.
To elucidate the role of Elf-1 in Fc epsilonRI alpha chain expression, rat Elf-1 cDNAs were isolated and characterized. The rat Elf-1 cDNA of 2744 bp contained an open reading frame of 1848 bp. In addition to the full length rat Elf-1 cDNA (named type 1), two splice isoforms were isolated. One of the two isoforms lacked the amino acid residues from 85th to 120th (type 2), and the other from 85th to 175th (type 3). Similar isoforms were also observed in human tissue. Overexpression of rat Elf-1 (type 1) using a transient coexpression system inhibited of the alpha chain promoter activity. The inhibition activity was different between the isoforms; the inhibition activity of type 2 was lower than that of type 1, and type 3 did not have an inhibitory effect. This observation suggested that each Elf-1 isoform played a different role in the gene expression under its control.
为阐明Elf-1在FcεRIα链表达中的作用，分离并鉴定了大鼠Elf-1 cDNA。2744 bp的大鼠Elf-1 cDNA包含一个1848 bp的开放阅读框。除了全长大鼠Elf-1 cDNA（命名为1型）外，还分离出两种剪接异构体。两种异构体中的一种缺少第85至120位氨基酸残基（2型），另一种缺少第85至175位氨基酸残基（3型）。在人类组织中也观察到类似的异构体。使用瞬时共表达系统过表达大鼠Elf-1（1型）抑制了α链启动子活性。异构体之间的抑制活性不同；2型的抑制活性低于1型，3型没有抑制作用。这一观察结果表明，每种Elf-1异构体在其控制下的基因表达中发挥不同的作用。
