Fletcher F A, Carpenter M K, Shilling H, Baum P, Ziegler S F, Gimpel S, Hollingsworth T, Vanden Bos T, James L, Hjerrild K
Immunex Research and Development Corporation, Seattle, Washington 98101.
Oncogene. 1994 Nov;9(11):3241-7.
We have isolated and characterized cDNA clones that encode the rat homologue of a binding protein, LERK-2, for the receptor tyrosine kinase, elk. The cDNAs contain an open reading frame of 1527 nucleotides capable of encoding a protein 345 amino acid residues in length. The nucleotide sequence of the present clones is > 90% identical to the previously identified human LERK-2 cDNA, and the predicted proteins encoded by the rat and human clones are identical at 95% of amino acid residues. Recombinant proteins expressed from the rat cDNAs bind to elk with high affinity, similar to recombinant human LERK-2 and an endogenously-expressed rat elk-binding protein. Expression of the rat LERK-2 mRNA was detected in embryonic brain, kidney, lung, skeletal muscle, thymus, liver, and heart, and diminished in the early post-natal period. Significant LERK-2 mRNA expression in the young adult rat was restricted to the lung, kidney, heart and testes.
我们已经分离并鉴定了编码大鼠同源结合蛋白（LERK-2）的cDNA克隆，该结合蛋白是受体酪氨酸激酶elk的结合蛋白。这些cDNA包含一个1527个核苷酸的开放阅读框，能够编码一个长度为345个氨基酸残基的蛋白质。目前克隆的核苷酸序列与先前鉴定的人类LERK-2 cDNA的同源性大于90%，大鼠和人类克隆所编码的预测蛋白质在95%的氨基酸残基处相同。从大鼠cDNA表达的重组蛋白与elk具有高亲和力结合，类似于重组人LERK-2和内源性表达的大鼠elk结合蛋白。在胚胎脑、肾、肺、骨骼肌、胸腺、肝和心脏中检测到大鼠LERK-2 mRNA的表达，并且在出生后早期减少。在成年大鼠中，显著的LERK-2 mRNA表达局限于肺、肾、心脏和睾丸。
