Braun K, Kuhl P, Bernd M, Kutscher B
Institute of Biochemistry, University of Technology Dresden, Germany.
Pharmazie. 2001 Jan;56(1):45-9.
In this study stabilities of several LHRH antagonists against proteolytic enzymes are compared. For the enzymatic tests 15 proteases which differ in both substrate specificity and pH optimum were selected. The cyclic and two linear antagonists proved to be extraordinarily stable against the enzymes used over an incubation time of 50 h. Some degradation products were identified by high-performance liquid chromatography combined with mass spectrometry.
在本研究中，比较了几种促黄体激素释放激素（LHRH）拮抗剂对蛋白水解酶的稳定性。对于酶促试验，选择了15种在底物特异性和最适pH方面存在差异的蛋白酶。环状和两种线性拮抗剂在50小时的孵育时间内对所用酶表现出极高的稳定性。通过高效液相色谱与质谱联用鉴定了一些降解产物。
