Bi Xiangdong, Shi Xiangyang, Baker James R
Department of Physical Sciences, Charleston Southern University, Charleston, SC 29406, USA.
J Biomater Sci Polym Ed. 2008;19(1):131-42. doi: 10.1163/156856208783227686.
Cancer targeting is crucial for cancer detection, therapy and targeted drug delivery. A dendrimer-peptide conjugate has been synthesized based on poly(amidoamine) dendrimer generation 5 (PAMAM G5) as a platform and a luteinizing hormone-releasing hormone (LHRH) peptide as a targeting moiety. The synthesized conjugate was fully characterized using nuclear magnetic resonance (NMR), UV-Vis spectrometry, reverse-phase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Further stability experiments showed that the synthesized conjugate was stable after 72-h incubation in phosphate-buffered saline (PBS) buffer (pH 7.4) at 37 degrees C. The synthesized conjugate may find applications in biomedical targeting, gene delivery and imaging.
癌症靶向对于癌症检测、治疗和靶向药物递送至关重要。基于第5代聚（酰胺胺）树枝状大分子（PAMAM G5）作为平台以及促黄体生成素释放激素（LHRH）肽作为靶向部分，合成了一种树枝状大分子 - 肽共轭物。使用核磁共振（NMR）、紫外 - 可见光谱法、反相高效液相色谱（RP - HPLC）和基质辅助激光解吸电离飞行时间（MALDI - TOF）质谱对合成的共轭物进行了全面表征。进一步的稳定性实验表明，合成的共轭物在37℃的磷酸盐缓冲盐水（PBS）缓冲液（pH 7.4）中孵育72小时后是稳定的。合成的共轭物可能在生物医学靶向、基因递送和成像方面找到应用。
