Melton T, Clifford S, Kayser M, Nasidze I, Batzer M, Stoneking M
Mitotyping Technologies, State College, PA 16801, USA.
J Forensic Sci. 2001 Jan;46(1):46-52.
Variation in the mitochondrial DNA (mtDNA) control region as detected by sequence-specific oligonucleotide (SSO) probes is described for 2282 individuals from African-American, European-American, and Hispanic subpopulations from five broadly defined regions of North America (Northeast, Southeast, Central, Northwest, Southwest). Population diversity estimates were uniformly high for all subpopulations and for each major ethnic group. Only the Pennsylvania Hispanic group was remarkable with respect to its mitochondrial DNA types, having both six low frequency population specific types (ranging from 1.2-8.6%) and three high frequency shared types (10-20% each). There was no statistically significant subpopulation heterogeneity present within any of the three major groups at either the subpopulation level or the regional level (p > 0.01). However, statistically significant heterogeneity was measured when comparing the three major groups to each other, with the variance component attributable to this large division accounting for 18.60% of the total variance (p < 0.001). Overall mtDNA is a satisfactory forensic typing locus within broadly defined African-American, European-American, and Hispanic groups from North America, based on the high diversity estimates and absence of heterogeneity, as characterized by SSO typing.
利用序列特异性寡核苷酸（SSO）探针检测了来自北美五个大致定义区域（东北、东南、中部、西北、西南）的非裔美国人、欧裔美国人及西班牙裔亚群体的2282名个体的线粒体DNA（mtDNA）控制区变异情况。所有亚群体及各主要种族群体的群体多样性估计值均普遍较高。仅宾夕法尼亚州的西班牙裔群体在其线粒体DNA类型方面较为显著，拥有六种低频群体特异性类型（范围为1.2 - 8.6%）和三种高频共享类型（各为10 - 20%）。在亚群体水平或区域水平上，三个主要群体中的任何一个群体内部均不存在统计学上显著的亚群体异质性（p > 0.01）。然而，在比较这三个主要群体时，测量到了统计学上显著的异质性，归因于这一较大分类的方差成分占总方差的18.60%（p < 0.001）。总体而言，基于高多样性估计值以及如SSO分型所表征的不存在异质性，mtDNA是北美大致定义的非裔美国人、欧裔美国人及西班牙裔群体内令人满意的法医分型位点。
