Korsgren O, Jansson L, Ekblad E, Sundler F
Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Sweden.
Transplantation. 2001 Jan 15;71(1):8-13. doi: 10.1097/00007890-200101150-00002.
Knowledge on the reinnervation of transplanted organs is scarce, and the aim of the study was therefore to evaluate to what degree syngeneic pancreas grafts were reinnervated in rats.
Syngeneic pancreatico-duodenal transplantations were performed in normoglycemic Wistar-Furth rats. Native and transplanted pancreas and duodenum were removed 4 or 40 weeks after implantation, and processed for indirect immunofluorescence using antibodies directed against vasoactive intestinal peptide, substance P (SP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), tyrosine hydroxylase (TH), or the general neuronal marker protein gene product 9.5.
Four weeks after transplantation a moderate to rich number of protein gene product 9.5-positive nerve fibers were found homogeneously distributed through the pancreas, probably representing the intrapancreatic nervous system, because the grafted pancreas lacked both a sympathetic (TH/NPY) and sensory (SP/CGRP) innervation 4 weeks after implantation. In a few of the animals there was a marked increase in SP-immunoreactive nerves (lacking CGRP), most conspicuous in the duodenal portion, both 4 and 40 weeks after transplantation probably secondary to a chronic pancreatitis. The fibers seemed to emanate from intrapancreatic ganglia and possibly also from enteric neurons in adjacent parts of the duodenum. A few scattered vasoactive intestinal peptide-containing nerve fibers probably also emanating from local ganglia could be seen throughout the grafted pancreas both 4 and 40 weeks after transplantation. At 40 weeks after transplantation sympathetic (TH- and NPY-positive) nerve fibers were regularly seen, whereas CGRP-positive nerve fibers were still virtually lacking in the pancreas. To trace the origin of the ingrowing nerve fibers, the tracer True Blue was injected into the grafted pancreas of some rats 38 weeks after transplantation, i.e., 2 weeks before killing. True Blue-labeled nerve cell bodies were numerous in the celiac ganglion (presumably sympathetic nerves) and few in dorsal root ganglia (sensory nerves).
The data suggest that the transplanted rat pancreas becomes reinnervated by mainly sympathetic nerve fibers.
关于移植器官再支配的知识匮乏，因此本研究的目的是评估同基因胰腺移植物在大鼠体内的再支配程度。
在血糖正常的Wistar-Furth大鼠中进行同基因胰十二指肠移植。在植入后4周或40周切除天然和移植的胰腺及十二指肠，并使用针对血管活性肠肽、P物质（SP）、降钙素基因相关肽（CGRP）、神经肽Y（NPY）、酪氨酸羟化酶（TH）或一般神经元标志物蛋白基因产物9.5的抗体进行间接免疫荧光处理。
移植后4周，在胰腺中发现中等至丰富数量的蛋白基因产物9.5阳性神经纤维均匀分布，可能代表胰腺内神经系统，因为植入后4周移植的胰腺缺乏交感神经（TH/NPY）和感觉神经（SP/CGRP）支配。在一些动物中，移植后4周和40周时，SP免疫反应性神经（缺乏CGRP）显著增加，在十二指肠部分最为明显，可能继发于慢性胰腺炎。这些纤维似乎起源于胰腺内神经节，也可能起源于十二指肠相邻部位的肠神经元。移植后4周和40周，在整个移植胰腺中都可见到一些可能也起源于局部神经节的散在含血管活性肠肽神经纤维。移植后40周，可见到交感神经（TH和NPY阳性）神经纤维，而胰腺中几乎仍缺乏CGRP阳性神经纤维。为了追踪长入神经纤维的起源，在移植后38周（即处死前2周）将示踪剂真蓝注入一些大鼠的移植胰腺。真蓝标记的神经细胞体在腹腔神经节（可能是交感神经）中很多，在背根神经节（感觉神经）中很少。
数据表明，移植的大鼠胰腺主要由交感神经纤维实现再支配。
