Sampaio J P, Gadanho M, Bauer R
Int J Syst Evol Microbiol. 2001 Jan;51(Pt 1):221-229. doi: 10.1099/00207713-51-1-221.
A new species of the genus Cystofilobasidium is described as Cystofilobasidium ferigula sp. nov. The new taxon represents the teleomorphic stage of Cryptococcus ferigula and was obtained in mating experiments using three strains deposited in the Portuguese Yeast Culture Collection (mating types A1) and a recent isolate (mating type A2). Cystofilobasidium ferigula is characterized using an integrated approach encompassing morphological studies, investigation of the ultrastructure of the septal pore, a comparative study of physiological traits, determination of the DNA base composition, DNA reassociation experiments and PCR fingerprinting. During the course of this study, a close similarity of microsatellite-primed PCR fingerprints was detected between Cystofilobasidium lari-marini and Cystofilobasidium capitatum. DNA-DNA reassociation experiments gave high homology values, which indicates that Cystofilobasidium lari-marini must be regarded as a synonym of Cystofilobasidium capitatum.
一种新的丝孢酵母属物种被描述为费氏丝孢酵母（Cystofilobasidium ferigula sp. nov.）。这个新分类单元代表了费氏隐球菌的有性型阶段，它是通过使用保存在葡萄牙酵母培养物保藏中心的三株菌株（交配型A1）和一株近期分离株（交配型A2）进行交配实验获得的。费氏丝孢酵母通过综合方法进行特征描述，包括形态学研究、隔膜孔超微结构研究、生理特性比较研究、DNA碱基组成测定、DNA重缔合实验和PCR指纹图谱分析。在这项研究过程中，检测到海滨丝孢酵母（Cystofilobasidium lari - marini）和头状丝孢酵母（Cystofilobasidium capitatum）之间微卫星引物PCR指纹图谱有密切相似性。DNA - DNA重缔合实验给出了高同源性值，这表明海滨丝孢酵母必须被视为头状丝孢酵母的同义词。
