Barrios Y, Plaza A, Cabrera R, Díaz-Espada F
Department of Immunology, Hospital Clínica Puerta de Hierro, Madrid, Spain.
APMIS. 2000 Nov;108(11):764-70. doi: 10.1034/j.1600-0463.2000.d01-27.x.
In patients with B-cell lymphoma, only in rare cases a secreted paraprotein is found, and in very few of them an associated autoantibody activity has been demonstrated. Here we report the case of a patient with a low-grade B-cell lymphoma with a serum biclonal paraprotein (G,M)lambda and severe erythroblastopenia. Indirect immunofluorescence studies of total serum revealed cytoplasmic (Hep-2 cells) and extracellular matrix (rat tissue sections) staining, suggestive of a new specificity. After gel filtration of serum samples, only the IgM-containing fraction showed the same pattern of staining. Tumor-derived hybridomas expressed an unmutated V3-11 gene identical to that found in tumor samples and secreted an IgM immunoglobulin endowed with the same reactivity, which confirms the tumoral origin of the tissue-reactive protein. The results suggest a link between the autoimmune condition in this patient and the novel specificity displayed by the tumor-derived immunoglobulin.
在B细胞淋巴瘤患者中，仅在极少数情况下可发现分泌型副蛋白，其中仅有极少数被证实存在相关自身抗体活性。在此，我们报告一例患有低度B细胞淋巴瘤的患者，其血清中存在双克隆副蛋白（G、M）λ以及严重的成红细胞减少症。对全血清进行的间接免疫荧光研究显示，在细胞质（Hep-2细胞）和细胞外基质（大鼠组织切片）中有染色现象，提示存在一种新的特异性。血清样本经凝胶过滤后，只有含IgM的部分呈现相同的染色模式。肿瘤来源的杂交瘤表达了一个与肿瘤样本中相同的未突变V3-11基因，并分泌出具有相同反应性的IgM免疫球蛋白，这证实了组织反应性蛋白的肿瘤来源。结果表明该患者的自身免疫状况与肿瘤来源免疫球蛋白所显示的新特异性之间存在联系。
