Kayser L, Francis D, Broholm H
Department of Medical Anatomy, Section A, Panum Institute, Copenhagen, Denmark.
APMIS. 2000 Nov;108(11):785-91. doi: 10.1034/j.1600-0463.2000.d01-30.x.
To investigate the distribution of nitric oxide synthase in tissues derived from patients with autoimmune or neoplastic disorders of the thyroid gland in order to test whether the expression of inducible nitric oxide synthase or endothelial constitutive nitric oxide synthase (two subtypes of EC 1.14.13.39) may be related to the inflammatory activity or degree of neoplasia.
The expression of nitric oxide synthases was examined by immunohistochemistry in tissues from patients with either Hashimoto's thyroiditis (n=6), hyperplastic glands (Graves' disease) (n=7), adenomas (n=8), multinodular goitres (n=7), papillary carcinomas (n=4) or follicular carcinomas (n=5).
Expression of inducible nitric oxide synthase was found in 22 of the tissues and was not specific for any of the examined thyroid disorders. Expression of endothelial constitutive nitric oxide synthase was found in some of the epithelial cells in all the tissues. There was no correlation between the intensity and distribution of the immunostaining and the thyroid disorders.
Demonstration of nitric oxide synthase cannot be used for diagnostic purposes. The expression of endothelial constitutive nitric oxide synthase in all tissues indicates that the enzyme may be of importance for the function or growth of the thyroid epithelial cells.
研究一氧化氮合酶在甲状腺自身免疫性疾病或肿瘤性疾病患者组织中的分布，以检验诱导型一氧化氮合酶或内皮型组成型一氧化氮合酶（EC 1.14.13.39的两种亚型）的表达是否与炎症活性或肿瘤形成程度相关。
采用免疫组织化学方法检测桥本甲状腺炎患者（n = 6）、增生性腺体（格雷夫斯病）患者（n = 7）、腺瘤患者（n = 8）、多结节性甲状腺肿患者（n = 7）、乳头状癌患者（n = 4）或滤泡状癌患者（n = 5）组织中一氧化氮合酶的表达。
在22个组织中发现了诱导型一氧化氮合酶的表达，且对任何一种所检测的甲状腺疾病均无特异性。在所有组织的一些上皮细胞中发现了内皮型组成型一氧化氮合酶的表达。免疫染色的强度和分布与甲状腺疾病之间无相关性。
一氧化氮合酶的检测不能用于诊断目的。所有组织中内皮型组成型一氧化氮合酶的表达表明该酶可能对甲状腺上皮细胞的功能或生长具有重要意义。
