Wang K, Wen Z, Zhang W, Xian M, Cheng J P, Wang P G
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Bioorg Med Chem Lett. 2001 Feb 12;11(3):433-6. doi: 10.1016/s0960-894x(00)00688-0.
Using UV-vis spectrometrical measurements, equilibrium constants for NO transfer between S-nitroso-N-acetyl-penicillamine (SNAP) and different thiols as well as kinetic data for NO transfer from S-nitroso bovine serum albumin (BSANO) to thiols have been obtained. NO transfer from SNAP to other primary/secondary thiols are thermodynamically favorable, whereas other S-nitrosothiols exhibit similar NO transfer potential. The obtained Gibbs free energy, enthalpy and entropy data indicated that NO transfer reactions from SNAP to four thiols are exothermic with entropy loss. The kinetic behavior of BSANO/RSH transfer can be related to both the acidity of sulfhydryl group and the electronic structure in thiol.
通过紫外可见光谱测量，已获得S-亚硝基-N-乙酰青霉胺（SNAP）与不同硫醇之间NO转移的平衡常数，以及NO从S-亚硝基牛血清白蛋白（BSANO）转移至硫醇的动力学数据。NO从SNAP转移至其他伯/仲硫醇在热力学上是有利的，而其他S-亚硝基硫醇表现出相似的NO转移潜力。所获得的吉布斯自由能、焓和熵数据表明，从SNAP到四种硫醇的NO转移反应是放热的且伴有熵损失。BSANO/RSH转移的动力学行为与巯基的酸度和硫醇中的电子结构均有关。
