Hirai K, Fujimura S, Shibata Y, Ishihara K, Kato T, Okuda K, Nakamura T
Department of Oral Microbiology, Matsumoto Dental University, 1780, Hirooka-Gobara, Shiojiri-shi, Nagano 399-0781, Japan.
Bull Tokyo Dent Coll. 2000 Aug;41(3):135-40. doi: 10.2209/tdcpublication.41.135.
We compared the effects of LPSs from P. gingivalis and P. heparinolytica on the induction of tumor necrosis factor-alpha (TNF-alpha) production by murine peritoneal macrophages. The lipopolysaccharide (LPS) preparation from P. gingivalis showed a typical ladder pattern in SDS-PAGE, whereas that from P. heparinolytica formed several stained bands without a ladder pattern. When the macrophages from C3H/HeJ mice were incubated with P. gingivalis LPS, the level of TNF-alpha released in the culture supernatants was significantly higher than that with P. heparinolytica. All tested reagents except genistein strongly inhibited the production of TNF-alpha by the macrophages after induction by either LPS. These results suggested the following possibilities, i) the induction level of TNF-alpha by P. heparinolytica is similar to that of Salmonella minnesota, ii) tyrosine phosphorylation is not the only pathway for the TNF-alpha production induced by these LPSs, iii) different regulatory mechanisms are involved in TNF-alpha production in P. gingivalis LPS stimulated and P. heparinolytica LPS stimulated cells.
我们比较了牙龈卟啉单胞菌和溶肝素卟啉单胞菌的脂多糖（LPS）对小鼠腹腔巨噬细胞诱导肿瘤坏死因子-α（TNF-α）产生的影响。牙龈卟啉单胞菌的脂多糖（LPS）制剂在SDS-PAGE中显示出典型的梯状模式，而溶肝素卟啉单胞菌的LPS制剂形成了几条染色带，没有梯状模式。当将C3H/HeJ小鼠的巨噬细胞与牙龈卟啉单胞菌LPS一起孵育时，培养上清液中释放的TNF-α水平明显高于与溶肝素卟啉单胞菌LPS孵育时。除染料木黄酮外，所有测试试剂在任何一种LPS诱导后均强烈抑制巨噬细胞产生TNF-α。这些结果提示了以下可能性：i）溶肝素卟啉单胞菌诱导TNF-α的水平与明尼苏达沙门氏菌相似；ii）酪氨酸磷酸化不是这些LPS诱导TNF-α产生的唯一途径；iii）牙龈卟啉单胞菌LPS刺激的细胞和溶肝素卟啉单胞菌LPS刺激的细胞中TNF-α产生涉及不同的调节机制。
