噬菌体λ在枯草芽孢杆菌核酸外切酶V存在下的生长与重组
Growth and recombination of phage lambda in the presence of exonuclease V from Bacillus subtilis.
作者信息
Stahl F, Bowers R, Mooney D, Myers R, Stahl M, Thomason L
机构信息
Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA.
出版信息
Mol Gen Genet. 2001 Jan;264(5):716-23. doi: 10.1007/s004380000361.
PMID:11212927
Abstract
When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage lambda. Mutants of lambda that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of lambda ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.
摘要
当在大肠杆菌中表达时，来自枯草芽孢杆菌的AddAB核酸外切酶/重组酶会阻断噬菌体λ的生长。λ的突变体缺失ea47（一个功能未知的基因，在裂解周期早期表达），其生长不会被阻断。只有在允许噬菌体DNA复制时，λ ea47+在AddAB存在下的生长阻断表型才会表现出来。
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