泌乳母羊乳腺游离多核糖体中酪蛋白mRNA的缺失。
Absence of mRNA for casein in free polysomes of lactating ewe mammary gland.
作者信息
Houdebine L M, Gaye P
出版信息
Nucleic Acids Res. 1975 Feb;2(2):165-78. doi: 10.1093/nar/2.2.165.
Abstract
Total polysomal RNA or poly A- containing RNA extracted from free polysomes of lactating ewe mammary gland was unable to direct a significant synthesis of alpha-s- and beta-casein. Polyacrylamide gel electrophoresis of poly A- containing RNA revealed that the two major peaks of mRNA isolated from membrane-bound polysomes were not observed in mRNA isolated from free polysomes. Purified 125-I anti-alpha-s-casein could bind significantly to membrane-bound polysomes only. These data suggest that casein synthesis occurs almost exclusively on membrane-bound polysomes and that this specificity may be attributed to the presence of casein mRNAs on these polysomes and their absence from free polysomes.
摘要
从泌乳母羊乳腺游离多核糖体中提取的总多核糖体RNA或含聚腺苷酸的RNA不能指导α-s-酪蛋白和β-酪蛋白的显著合成。对含聚腺苷酸的RNA进行聚丙烯酰胺凝胶电泳显示，从膜结合多核糖体中分离出的mRNA的两个主要峰在从游离多核糖体中分离出的mRNA中未观察到。纯化的125-I抗α-s-酪蛋白只能与膜结合多核糖体显著结合。这些数据表明，酪蛋白合成几乎只发生在膜结合多核糖体上，这种特异性可能归因于这些多核糖体上存在酪蛋白mRNA，而游离多核糖体上不存在。
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本文引用的文献
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Nucleic Acids Res. 1974 Mar;1(3):413-26. doi: 10.1093/nar/1.3.413.
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Ribosomes in rat liver: an estimate of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivo.大鼠肝脏中的核糖体：体内游离核糖体和膜结合核糖体与信使核糖核酸相互作用的百分比估算
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