Zhou X, Lu Z, Zhao H, Wang D
Department of Internal Medicine, Tongji Hospital, Tongji Medical University, Wuhan 430030.
J Tongji Med Univ. 2000;20(3):205-7. doi: 10.1007/BF02886990.
To evaluate the possibility of employing ligustrazine in the prevention of restenosis, the effects of ligustrazine on the intimal thickening of air-injured carotid artery of rats were investigated, and the effects of ligustrazine on the proliferation of rabbit aortic median smooth muscle cells (SMCs) cultivated in vitro were examined. Artery injury model of 18 rats of about 3 months old was established by Fishman air-dry method. Fourteen days after operation, the maximal artery intimal and medial thickness of the control and ligustrazine group was measured on the image analysis system. Using cell counting and thymidine (3H-TdR) up-take method, we also examined the effects of ligustrazine on the proliferation of aortic median SMC from 4 rabbits. Ligustrazine was found to inhibit the proliferation and 3H-TdR up-take of SMC in a dose-dependent manner in vitro (P < 0.05 or P < 0.01 vs control). It also inhibited the intimal thickening of rat arteries after deendothelialization. The maximal intimal thickness of ligustrazine group was much thinner than that of the control (35.9 +/- 3.8 microns vs 80.2 +/- 23.4 microns, P < 0.01). It was showed that ligustrazine could be used for prevention of restenosis in clinical practice.
为评估应用川芎嗪预防再狭窄的可能性，研究了川芎嗪对大鼠空气损伤颈动脉内膜增厚的影响，并检测了川芎嗪对体外培养的兔主动脉中膜平滑肌细胞（SMC）增殖的影响。采用Fishman空气干燥法建立18只3月龄左右大鼠的动脉损伤模型。术后14天，在图像分析系统上测量对照组和川芎嗪组的动脉最大内膜和中膜厚度。我们还采用细胞计数和胸腺嘧啶核苷（3H-TdR）掺入法，检测了川芎嗪对4只兔主动脉中膜SMC增殖的影响。结果发现，川芎嗪在体外能以剂量依赖方式抑制SMC的增殖和3H-TdR掺入（与对照组相比，P<0.05或P<0.01）。它还能抑制去内皮大鼠动脉的内膜增厚。川芎嗪组的最大内膜厚度比对照组薄得多（35.9±3.8微米对80.2±23.4微米，P<0.01）。结果表明，川芎嗪可用于临床预防再狭窄。
