Li S, Wang J H, Chen S L
Emergency Center, First Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine and Pharmacology, Guangzhou 510407, China.
Zhongguo Yao Li Xue Bao. 1999 Oct;20(10):917-22.
To study the inhibitory effect of ligustrazine (Lig) on growth of cultured rabbit aortic vascular smooth muscle cells (VSMC) after balloon injury.
Twenty New Zealand white rabbits were subjected to arterial injury with a balloon catheter (810 kPa for three consecutive inflations, 1 min each time). The uptake of [3H]thymidine in primary cultural VSMC incubated with rabbit serum, which obtained from the animals treated without or with Lig (40 mg.kg-1.d-1, i.v.) for 21 d (7 d before and 14 d after the injury procedure) was determined. The determination was performed in direct addition of TMP to culture as well. And histological cross-sections of the blood wall were also analyzed.
After balloon injury the intimal thickening (77 +/- 23) microns and lumen diameter narrowing (877 +/- 118) microns in dilated sites were increased significantly than the normal adjacent wall [(41 +/- 13) microns, P < 0.01; (1033 +/- 175) microns, P < 0.05, respectively]. Treatment with Lig decreased both intimal thickening (56 +/- 16) microns (P < 0.05) and lumen diameter narrowing (1023 +/- 157) microns (P < 0.05). Lig inhibited [3H]thymidine uptake in VSMC incubated with the serum obtained from these rabbits. Direct addition of Lig inhibited [3H]thymidine uptake in cultured VSMC in a dose-dependent manner (40-4000) micrograms/well.
Lig shows a pronounced inhibitory effect on VSMC proliferation after balloon injury.
研究川芎嗪（Lig）对球囊损伤后培养的兔主动脉血管平滑肌细胞（VSMC）生长的抑制作用。
20只新西兰白兔用球囊导管进行动脉损伤（810 kPa连续充气3次，每次1分钟）。测定用未经处理或经Lig（40 mg·kg-1·d-1，静脉注射）处理21天（损伤前7天和损伤后14天）的动物血清培养的原代VSMC中[3H]胸腺嘧啶核苷的摄取。也通过直接向培养物中添加胸苷酸进行测定。并且还分析了血管壁的组织学横截面。
球囊损伤后，扩张部位的内膜增厚（77±23）微米和管腔直径变窄（877±118）微米比正常相邻壁显著增加[（41±13）微米，P<0.01；（1033±175）微米，P<0.05]。Lig治疗可减少内膜增厚（56±16）微米（P<0.05）和管腔直径变窄（1023±157）微米（P<0.05）。Lig抑制用这些兔子血清培养的VSMC中[3H]胸腺嘧啶核苷的摄取。直接添加Lig以剂量依赖性方式（40-4000）微克/孔抑制培养的VSMC中[3H]胸腺嘧啶核苷的摄取。
Lig对球囊损伤后VSMC增殖有明显的抑制作用。
