Hoshino H, Hino K, Sainokami S, Miyakawa H
Hepatitis Research Institute, Suginami-ku, Tokyo 167-0033.
Rinsho Byori. 2000 Dec;48(12):1168-74.
The clinical utility of the TMA-HPA method for the detection of HBV DNA was evaluated by comparing results with the branched DNA probe(b-DNA) assay. Sera from 135 biopsy-confirmed HBV carriers were included in the study. Dilution tests with two representative sera revealed the sensitivity of the TMA-HPA method to be 100-fold higher than that of the b-DNA assay. Among the 135 test sera, HBV DNA measurements obtained by the two assays showed a high degree of correlation(r = 0.861, p < 0.0001). Of the 72 sera which tested positive for HBe antigen, 71 and 67 were positive for HBV DNA when tested by TMA-HPA and b-DNA assay, respectively. In contrast, of the 63 which tested negative for HBe antigen, 42 were positive for HBV DNA when tested by TMA-HPA, and only 19 were positive when tested by b-DNA assay(p < 0.05). Among HBe antigen-positive HBV carriers, HBV DNA levels as determined both TMA-HPA and b-DNA assay showed an inverse relationship with pathological stage, but among HBe antigen-negative HBV carriers, no such tendency was observed. Based on these results, it is felt that the detection of HBV DNA by TMA-HPA is useful for the evaluating anti-viral therapeutic effect in HBV carriers, and in estimating a prognosis.
通过将检测结果与分支DNA探针（b-DNA）检测法进行比较，评估了TMA-HPA法检测HBV DNA的临床实用性。135例经活检确诊的HBV携带者的血清被纳入该研究。对两份代表性血清进行的稀释试验表明，TMA-HPA法的灵敏度比b-DNA检测法高100倍。在135份检测血清中，两种检测方法测得的HBV DNA结果显示出高度相关性（r = 0.861，p < 0.0001）。在72份HBe抗原检测呈阳性的血清中，通过TMA-HPA法和b-DNA检测法检测时，分别有71份和67份HBV DNA呈阳性。相比之下，在63份HBe抗原检测呈阴性的血清中，通过TMA-HPA法检测时，有42份HBV DNA呈阳性，而通过b-DNA检测法检测时，只有19份呈阳性（p < 0.05）。在HBe抗原阳性的HBV携带者中，TMA-HPA法和b-DNA检测法测定的HBV DNA水平均与病理分期呈负相关，但在HBe抗原阴性的HBV携带者中未观察到这种趋势。基于这些结果，认为通过TMA-HPA法检测HBV DNA有助于评估HBV携带者的抗病毒治疗效果和估计预后。
