Uchida K, Nakanishi M, Yoh K
FALCO Biosystems Co., Kuze-gun, Kyoto-pref. 613-0036.
Rinsho Byori. 2001 Jan;49(1):51-5.
Band 5 Tartrate-resistant acid phosphatase(TRACP; EC 3.1.3.2) consists of two isoenzymes, bands 5a and 5b, of which band 5b TRACP, an enzyme expressed in bone-resorbing osteoclasts, is secreted into the circulation during bone resorption. Band 5b TRACP was measured kinetically in serum as tartrate-resistant fluoride-sensitive heparin-resistant ACP with 2,6-dichloro-4-acetylphenyl phosphate as substrate at pH6.6. The within-run(n = 20) and between-run(n = 20) CVs of band 5b TRACP activity were 3.3-5.8% and 5.0-7.3%, respectively. The reference range of band 5b TRACP activity in males(n = 72) and females(n = 87) 20-39 years of age by this method were 3.7-12.5 U/l and 2.7-9.9 U/l, respectively. The band 5b TRACP value was significantly higher in post-menopausal women compared with the menstruating women. The relationship of band 5b TRACP and ultrasound findings in healthy women aged 31-75 years(n = 139) were inversely correlated with stiffness(r = -0.401), speed of sound(SOS; r = -0.386) and broadband ultrasound attenuation(BUA; r = -0.338). These results suggest that band 5b TRACP may be a useful in the evaluation of bone turnover.
5b型抗酒石酸酸性磷酸酶（TRACP；EC 3.1.3.2）由两种同工酶组成，即5a和5b条带，其中5b条带TRACP是一种在骨吸收破骨细胞中表达的酶，在骨吸收过程中分泌到循环系统中。以2,6-二氯-4-乙酰基苯磷酸为底物，在pH6.6条件下，将血清中的5b条带TRACP作为抗酒石酸、氟敏感、肝素抵抗的酸性磷酸酶进行动力学测定。5b条带TRACP活性的批内（n = 20）和批间（n = 20）变异系数分别为3.3 - 5.8%和5.0 - 7.3%。采用该方法测定的20 - 39岁男性（n = 72）和女性（n = 87）的5b条带TRACP活性参考范围分别为3.7 - 12.5 U/l和2.7 - 9.9 U/l。绝经后女性的5b条带TRACP值显著高于月经周期女性。在31 - 75岁的健康女性（n = 139）中，5b条带TRACP与超声检查结果的关系与硬度（r = -0.401）、声速（SOS；r = -0.386）和宽带超声衰减（BUA；r = -0.338）呈负相关。这些结果表明，5b条带TRACP可能在评估骨转换方面具有一定作用。
