Hui F, Ekborg-Ott K H, Armstrong D W
Department of Chemistry, University of Missouri-Rolla, 65409, USA.
J Chromatogr A. 2001 Jan 12;906(1-2):91-103. doi: 10.1016/s0021-9673(00)00954-7.
Enantioseparation of plant growth regulators, such as 3-(3-indolyl)-butyric acid, abscisic acid and structurally related molecules including a variety of substituted tryptophan compounds, has been achieved by HPLC and/or CE. The covalently bonded macrocyclic antibiotics, teicoplanin, ristocetin A and vancomycin, were used as chiral stationary phases (CSPs) in HPLC. Most of the racemates were baseline resolved in the reversed-phase mode (EtOH-H2O) using the teicoplanin CSP. The chiral recognition mechanism is discussed in regard to the structure of the analytes. In CE, the three aforementioned macrocyclic antibiotics were used as chiral additives in a phosphate run buffer. The effect of pH and the concentration of the organic modifiers were considered. The results obtained by HPLC and CE were compared.
通过高效液相色谱法（HPLC）和/或毛细管电泳（CE）已实现了植物生长调节剂（如3-(3-吲哚基)-丁酸、脱落酸）以及包括各种取代色氨酸化合物在内的结构相关分子的对映体拆分。共价键合的大环抗生素替考拉宁、瑞斯托菌素A和万古霉素在HPLC中用作手性固定相（CSP）。使用替考拉宁CSP时，大多数外消旋体在反相模式（乙醇-水）下实现了基线分离。针对分析物的结构对手性识别机制进行了讨论。在CE中，上述三种大环抗生素在磷酸盐运行缓冲液中用作手性添加剂。考虑了pH值和有机改性剂浓度的影响。比较了HPLC和CE获得的结果。
