Bogomolova N N, Shukhmina N R, Andzhaparidze O G
Vopr Virusol. 1975 Jan-Feb(1):71-4.
Studies on cloning of continuous HEp-2 and SPEV cell lines were carried out. The sensitivity of the resulting clones to tick-borne encephalitis virus was determined and the clone lines were shown to be heterologous in their sensitivity to TBE virus by means of the immunofluorescence and virological methods. Among 47 clones of HEp-2 line virus reproduction was observed in 30 clones, no reproduction was demonstrated in 17 clones. The maximum number of cells simultaneously synthesizing virus antigen did not exceed 35% of the population which was conformity with the results obtained in the study of the original HEp-2 cell line. Twenty-nine clones derived from the continuous SPEV cell line were examined. Reproduction of the virus was observed in all of them. However, according to the maximum number of cells involved in the process of antigen synthesis, all the clones could be divided into two groups which differed also by the dynamics of cell involvement in antigen synthesis. The results of the study of clones derived from chronically TBE-infected Hep-2-Soph cell line are presented. In 13 out of 15 clones, the infectious virus and antigen synthesis were demonstrated which suggested that the majority of cells of the parental HEp-2-Soph line had been infected with TBE virus.
开展了连续的HEp-2和SPEV细胞系的克隆研究。测定了所得克隆对蜱传脑炎病毒的敏感性，并通过免疫荧光和病毒学方法证明克隆系对蜱传脑炎病毒的敏感性存在异质性。在HEp-2细胞系的47个克隆中，30个克隆观察到病毒增殖，17个克隆未显示病毒增殖。同时合成病毒抗原的细胞的最大数量不超过细胞总数的35%，这与原始HEp-2细胞系研究中获得的结果一致。检测了来自连续SPEV细胞系的29个克隆。在所有克隆中均观察到病毒增殖。然而，根据参与抗原合成过程的细胞的最大数量，所有克隆可分为两组，这两组在细胞参与抗原合成的动态方面也有所不同。给出了来自慢性感染蜱传脑炎病毒的Hep-2-Soph细胞系的克隆的研究结果。在15个克隆中的13个克隆中，证明了感染性病毒和抗原的合成，这表明亲本Hep-2-Soph细胞系的大多数细胞已感染蜱传脑炎病毒。
