Andzhaparidze O G, Bogomolova N N, Boriskin Iu S, Desiatskova R G, Bektemirova M S
Vopr Virusol. 1980 May-Jun(3):323-7.
A comparative study of chronic infecton of HEp-2 cells with tick-borne encephalitis (TBE), rabies (RV), and rubella (RuV) viruses was carried out. Throughout the entire period of chronic infection (CI) no signs of specific cell destruction by these viruses were observed. The infectious virus was regularly demonstrated in the culture fluid and chronically infected cells. The antigenic properties of the persisting viruses did not differ from those of the original strains. The persisting TBE and rabies viruses replicated in the susceptible cells at a higher temperature and formed plaques of a smaller size than the original virus. The number of chronically infected cells producing infectious virus was always less than the number of cells containing the virus-specific antigen. In all three types of chronic infection the cells supported virus persistence at 40 degrees C. In TBE and RuV chronically infected cells interference with heterologous viruses was marked while in HEp-2-RV homologous interference caused by formation of defective interfering particles was observed. Treatment of the cells with BUDR resulted in activation of the infection only in the HEp-2-TBE system. Experiments on transfection of the sensitive cells by using DNA from HEp-2-RV and HEp-2-RuV gave negative results. The importance of various factors in the mechanism of virus persistence in the chronically infected cells under study is discussed.
对蜱传脑炎病毒（TBE）、狂犬病病毒（RV）和风疹病毒（RuV）慢性感染人喉表皮样癌细胞（HEp - 2）进行了一项比较研究。在整个慢性感染（CI）期间，未观察到这些病毒对细胞有特异性破坏的迹象。在培养液和慢性感染细胞中经常检测到感染性病毒。持续存在的病毒的抗原特性与原始毒株无异。持续存在的TBE病毒和狂犬病病毒在较高温度下能在易感细胞中复制，且形成的蚀斑比原始病毒形成的蚀斑小。产生感染性病毒的慢性感染细胞数量始终少于含有病毒特异性抗原的细胞数量。在所有三种慢性感染类型中，细胞在40℃时能维持病毒持续存在。在TBE和RuV慢性感染细胞中，对异源病毒的干扰很明显，而在HEp - 2 - RV中，观察到由缺陷干扰颗粒形成引起的同源干扰。用溴脱氧尿苷（BUDR）处理细胞仅在HEp - 2 - TBE系统中导致感染激活。用来自HEp - 2 - RV和HEp - 2 - RuV的DNA转染敏感细胞的实验结果为阴性。讨论了各种因素在研究的慢性感染细胞中病毒持续存在机制中的重要性。
