Wahlfors J, Loimas S, Pasanen T, Hakkarainen T
Gene Transfer Technology Group, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, Finland.
Histochem Cell Biol. 2001 Jan;115(1):59-65. doi: 10.1007/s004180000219.
The history of green fluorescent protein (GFP) as a marker is less than 10 years old, but it has already made a major impact on many areas of natural sciences, especially on cell biology and histochemistry. GFP can be detected in living cells without selection or staining and it can be fused to other proteins to yield fluorescent chimeras. The potential of GFP has also been recognised by gene therapy researchers and various GFP-tagged therapeutic proteins have been constructed. These chimeric proteins have been used to determine the expression level, site and time course of the therapeutic gene, or the correlation between gene transfer rate and therapeutic outcome. This review summarises the status of the applications of GFP fusions in gene therapy research.
绿色荧光蛋白（GFP）作为一种标记物的历史还不到10年，但它已经对自然科学的许多领域产生了重大影响，尤其是细胞生物学和组织化学领域。无需筛选或染色即可在活细胞中检测到GFP，并且它可以与其他蛋白质融合以产生荧光嵌合体。基因治疗研究人员也认识到了GFP的潜力，并且已经构建了各种带有GFP标签的治疗性蛋白质。这些嵌合蛋白已被用于确定治疗基因的表达水平、位点和时间进程，或基因转移率与治疗效果之间的相关性。本综述总结了GFP融合体在基因治疗研究中的应用现状。
