Pérez E, Sawyers W G, Martin D F
Department of Chemistry, University of South Florida, Tampa 33620, USA.
Cytobios. 2001;104(405):25-31.
Laboratory cultures of Florida's red tide organism, Gymnodinium breve, were killed by the green alga Nannochloris eucaryotum. Studies involved organism-organism interaction as well as organism-cell-free culture (N. eucaryotum) interaction. Both studies demonstrated that N. eucaryotum adversely affected Florida's red tide organism. The lysis has been attributed to compounds called APONINs (apparent oceanic naturally occurring cytolins). N. eucaryotum crude APONIN was extracted from cell-free cultures, partially purified and fractionated. The fractions were bioassayed against G. breve, and 'fingerprints' of the deleterious fractions were obtained.
佛罗里达赤潮生物——短裸甲藻（Gymnodinium breve）的实验室培养物被绿藻真核小球藻（Nannochloris eucaryotum）杀死。研究涉及生物体与生物体之间的相互作用以及生物体与无细胞培养物（真核小球藻）之间的相互作用。两项研究均表明，真核小球藻对佛罗里达赤潮生物有不利影响。这种裂解归因于一种名为APONINs（明显的海洋天然存在的细胞溶素）的化合物。从无细胞培养物中提取真核小球藻粗APONIN，进行部分纯化和分级分离。将这些级分针对短裸甲藻进行生物测定，得到了有害级分的“指纹图谱”。
