Akimoto T, Sasaki S, Ikebukuro K, Karube I
Research Center for Advanced Science and Technology, The University of Tokyo, Japan.
Biosens Bioelectron. 2000 Oct;15(7-8):355-62. doi: 10.1016/s0956-5663(00)00091-9.
The effect of the incident angle of light on sensitivity and the detection limit for surface-plasmon resonance spectroscopy were examined. The sensitivities and the detection limit were experimentally measured using an antibody as a modeled analyte in the incident angles of a light region of 66-76 degrees. The results showed that the sensitivity of a smaller incident angle was higher than that of a larger one. For instance, the sensitivity of a 66 degree incident angle was three times higher than that of a 76 degree incident angle. The detection limit with a 66 degree incident angle was one-tenth of that with a 76 degree incident angle. These sensitivities and detection limits were compared with those of a commercially produced surface-plasmon resonance instrument. This comparison demonstrated that a wavelength resolution of the order of less than 10(-2) nm was necessary to obtain satisfactory sensitivities and detection limits. In addition, the refractive index and thickness of the antibody layer formed on a sensor surface was proposed by experimental results and theoretical calculation.
研究了光入射角对表面等离子体共振光谱灵敏度和检测限的影响。在66 - 76度光区域的入射角下，以抗体作为模拟分析物，通过实验测量了灵敏度和检测限。结果表明，较小入射角的灵敏度高于较大入射角的灵敏度。例如，66度入射角的灵敏度是76度入射角灵敏度的三倍。66度入射角的检测限是76度入射角检测限的十分之一。将这些灵敏度和检测限与商业生产的表面等离子体共振仪器的灵敏度和检测限进行了比较。这种比较表明，要获得令人满意的灵敏度和检测限，波长分辨率需小于10(-2)nm量级。此外，通过实验结果和理论计算提出了传感器表面形成的抗体层的折射率和厚度。
