Yuk Jong Seol, Hong Duk-Geun, Jung Jae-Wan, Jung Se-Hui, Kim Hyun-Soo, Han Jeong-A, Kim Young-Myeong, Ha Kwon-Soo
Department of Molecular and Cellular Biochemistry and Nano-Bio Sensor Research Center, Kangwon National University School of Medicine, Chunchon, Kangwon-Do, 200-701, South Korea.
Eur Biophys J. 2006 Aug;35(6):469-76. doi: 10.1007/s00249-006-0054-x. Epub 2006 Apr 7.
A novel method for sensitivity enhancement of spectral surface plasmon resonance (SPR) biosensors was presented by reducing the refractive index of the sensing prism in the analysis of protein arrays. Sensitivity of spectral SPR biosensors with two different prisms (BK-7, fused silica) was analyzed by net shifts of resonance wavelength for specific interactions of GST-GTPase binding domain of p21-activated kinase-1 and anti-GST on a mixed thiol surface. Sensitivity was modulated by the refractive index of the sensing prism of the spectral SPR biosensors with the same incidence angle. The sensitivity of a spectral SPR biosensor with a fused silica prism was 1.6 times higher than that with a BK-7 prism at the same incidence angle of 46.2 degrees. This result was interpreted by increment of the penetration depth correlated with evanescent field intensity at the metal/dielectric interface. Therefore, it is suggested that sensitivity enhancement is readily achieved by reducing the refractive index of the sensing prism of spectral SPR biosensors to be operated at long wavelength ranges for the analysis of protein arrays.
在蛋白质阵列分析中，通过降低传感棱镜的折射率，提出了一种提高光谱表面等离子体共振（SPR）生物传感器灵敏度的新方法。通过在混合硫醇表面上p21激活激酶-1的GST-GTPase结合域与抗GST的特异性相互作用所导致的共振波长净位移，分析了具有两种不同棱镜（BK-7、熔融石英）的光谱SPR生物传感器的灵敏度。在相同入射角下，光谱SPR生物传感器的灵敏度由传感棱镜的折射率调节。在46.2度的相同入射角下，具有熔融石英棱镜的光谱SPR生物传感器的灵敏度比具有BK-7棱镜的传感器高1.6倍。该结果通过与金属/电介质界面处倏逝场强度相关的穿透深度的增加来解释。因此，建议通过降低光谱SPR生物传感器的传感棱镜的折射率，以便在长波长范围内操作来分析蛋白质阵列，从而很容易实现灵敏度的提高。
