Zhao W, Devamanoharan P S, Henein M, Ali A H, Varma S D
Department of Ophthalmology, School of Medicine, University of Maryland, Baltimore 21201, USA.
Diabetes Obes Metab. 2000 Jun;2(3):165-74. doi: 10.1046/j.1463-1326.2000.00079.x.
Studies have been conducted to determine the effect of pyruvate administration on the biochemistry of rat lens and the status of its transparency as affected by diabetic conditions.
Sprague-Dawley rats were rendered diabetic by intravenous (i.v.) injection of streptozotocin (55 mg/kg body weight (b.w.)) and treated with sodium pyruvate (2%) in drinking water. The levels of glucose, fructose, sorbitol, ATP, GSH, MDA as well as glycated proteins in the lenses were determined at various intervals after the onset of diabetes and the values compared with untreated diabetic controls. The progress of cataract formation and associated histological changes in the tissue were also monitored.
Studies show that the pyruvate treatment decreased the extent of several biochemical changes known to be associated with cataract formation, such as the elevation in the levels of glycated proteins, sorbitol, lipid peroxidation (MDA) and inhibition of the cation pump. The progress of cataract was also significantly delayed.
Exogenous administration of this compound hence was found to exert an overall protective effect against cataract formation induced by the diabetic conditions.
已开展多项研究以确定丙酮酸给药对大鼠晶状体生物化学的影响以及糖尿病状况对其透明度状态的影响。
通过静脉注射链脲佐菌素（55毫克/千克体重）使斯普拉格-道利大鼠患糖尿病，并用饮用水中的丙酮酸钠（2%）进行治疗。在糖尿病发病后的不同时间间隔测定晶状体中葡萄糖、果糖、山梨醇、三磷酸腺苷、谷胱甘肽、丙二醛以及糖化蛋白的水平，并将这些值与未治疗的糖尿病对照组进行比较。还监测了白内障形成的进展以及组织中的相关组织学变化。
研究表明，丙酮酸治疗降低了几种已知与白内障形成相关的生化变化程度，如糖化蛋白、山梨醇水平升高、脂质过氧化（丙二醛）以及阳离子泵抑制。白内障的进展也显著延迟。
因此发现外源性给予该化合物对糖尿病诱导的白内障形成具有总体保护作用。
