David J C, Landry J, Grongnet J F
Laboratoire de Sciences Animales, Ecole Nationale Supérieure Agronomique, Rennes, France.
J Mol Neurosci. 2000 Oct;15(2):109-20. doi: 10.1385/jmn:15:2:109.
Important stressful events occur at birth or within the few hours that follow this event. To unravel a possible involvement of stress proteins, it is important to determine their levels of expression. Expression of HSP27 protein was determined by Western blotting in several nonneural tissues and in brain regions of the developing pig (fetal to adult). Maximum levels of expression were observed in heart, liver, and lung. In kidney, the expression was reduced during the first hours of life. Brain parts included whole brain, cerebellum, cortex, hippocampus, hypothalamus, and striatum. HSP27 immunogenicity was observed in all the brain regions studied. In whole brain, cerebellum, cortex, hippocampus, and hypothalamus extracts, the levels of HSP27 were decreased during the first hours of age (4 h to 2 d). In striatum, levels of expression were very low--if detectable--during the early postnatal days of life. Changes in environmental parameters, like temperature and/or hypoxia can be possibly related to differential expressions of HSPs, which can result in severe adverse developmental outcomes. The results are discussed in terms of using the newborn piglet as a model to study different forms of stress on the heat-shock protein postnatal expression.
重要的应激事件发生在出生时或出生后的几个小时内。为了阐明应激蛋白可能的作用，确定其表达水平很重要。通过蛋白质印迹法测定了发育中的猪（从胎儿到成年）的几种非神经组织和脑区中HSP27蛋白的表达。在心脏、肝脏和肺中观察到最高表达水平。在肾脏中，出生后的最初几个小时内表达降低。脑区包括全脑、小脑、皮质、海马体、下丘脑和纹状体。在所研究的所有脑区中均观察到HSP27的免疫原性。在全脑、小脑、皮质、海马体和下丘脑提取物中，HSP27的水平在出生后的最初几个小时（4小时至2天）内降低。在纹状体中，出生后早期的表达水平非常低（如果可检测到的话）。环境参数的变化，如温度和/或缺氧，可能与热休克蛋白的差异表达有关，这可能导致严重的不良发育结果。本文以新生仔猪为模型，探讨了不同形式的应激对热休克蛋白出生后表达的影响，并对结果进行了讨论。
