David J C, Grongnet J F
Laboratoire des Sciences Animales, Ecole Nationale Supérieure Agronomique, Rennes, France.
Biol Neonate. 2001 Feb;79(2):131-9. doi: 10.1159/000047079.
Important stressful events occur at birth or within the few hours that follow. To establish a possible involvement of stress proteins, expression of heat-shock protein 90 was determined by Western blotting in several regions of the brain and in non-neural tissues of the developing piglet (fetal to 10 days and adult). Expression was found in all the tissues studied. While comparable values were found in the whole brain during development, decreased expressions were observed from 4 to 8 h to 2 days after birth in cerebellum, cortex, hypothalamus and striatum. In hippocampus, low expression was observed from 4 h postnatally onward. In non-neural tissues, low expression was observed after birth and in the adult for heart, liver and lungs. In kidney, low values were found from birth to 1 day of age. Changes in environmental parameters like temperature and/or hypoxia can be related to differential expressions of heat-shock proteins and they possibly result in severe developmental outcomes. The results are discussed in terms of using the newborn piglet as a model for the study of different forms of stress on the heat-shock protein expression during postnatal development.
重要的应激事件发生在出生时或随后的几个小时内。为了确定应激蛋白可能的参与情况，通过蛋白质印迹法测定了发育中小猪（胎儿至10日龄及成年）大脑的几个区域和非神经组织中热休克蛋白90的表达。在所研究的所有组织中均发现了表达。虽然在发育过程中全脑中的表达值相当，但在出生后4至8小时到2天，小脑、皮质、下丘脑和纹状体中的表达下降。在海马体中，出生后4小时起观察到低表达。在非神经组织中，心脏、肝脏和肺在出生后及成年期观察到低表达。在肾脏中，从出生到1日龄发现表达值较低。温度和/或缺氧等环境参数的变化可能与热休克蛋白的差异表达有关，并且它们可能导致严重的发育结果。就将新生小猪作为研究产后发育过程中不同形式应激对热休克蛋白表达影响的模型而言，对结果进行了讨论。
