通过阴离子色谱法/电感耦合等离子体质谱法对细胞提取物中的锑（III）和锑（V）进行形态分析。
Speciation of antimony(III) and antimony(V) in cell extracts by anion chromatography/inductively coupled plasma mass spectrometry.
作者信息
Ulrich N, Shaked P, Zilberstein D
机构信息
University of Hannover, Department of Inorganic Chemistry, Germany.
出版信息
Fresenius J Anal Chem. 2000 Sep;368(1):62-6. doi: 10.1007/s002160000441.
An analytical method for the separation and quantification of Sb(III) and Sb(V) using anion chromatography with ICP-MS is presented. The optimum conditions for the separation of the antimony species were established with 15 mmol/L nitric acid at pH 6 as eluent system on a PRP-X100 column. The retention times for antimony(V) and antimony(III) were 85 s and 300 s with detection limits of 0.06 microg/L and 0.29 microg/L, respectively. The proposed method was applied to cell extracts of Leishmania donovani, which were incubated with antimony(III) and antimony(V). Some metabolism seemed to occur within the cells.
介绍了一种使用阴离子色谱-电感耦合等离子体质谱法分离和定量测定Sb(III)和Sb(V)的分析方法。在PRP-X100柱上，以pH 6的15 mmol/L硝酸为洗脱液体系，确定了锑形态分离的最佳条件。锑(V)和锑(III)的保留时间分别为85 s和300 s，检测限分别为0.06 μg/L和0.29 μg/L。该方法应用于杜氏利什曼原虫的细胞提取物，这些提取物与锑(III)和锑(V)一起孵育。细胞内似乎发生了一些代谢过程。
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