Lin Ya-An, Jiang Shiuh-Jen, Sahayam A C
Department of Chemistry, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
Department of Chemistry, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Food Chem. 2017 Sep 1;230:76-81. doi: 10.1016/j.foodchem.2017.03.014. Epub 2017 Mar 6.
A method was developed by coupling ion chromatography (IC) and inductively coupled plasma mass spectrometry (ICP-MS) for the speciation of antimony. In this study, antimony species such as antimonite [Sb(III)], antimonate [Sb(V)] and trimethyl antimony(V) (TMeSb) were separated in less than 8min using anion exchange chromatography with a Hamilton PRP-X100 column as the stationary phase. Mobile phase A was 20mmolL ethylenediaminetetraacetic acid (EDTA), 2mmolL potassium hydrogen phthalate (KHP) in 1% v/v methanol (pH 5.5) and 20mmolL EDTA, 2mmolL KHP, 40mmolL (NH)CO in 1% v/v methanol (pH 9.0) formed mobile phase B. Detection limits and relative standard deviations (RSD) were 0.012-0.032ngmL and 2.2-2.8% respectively. This method was applied to bottled waters and fruit juices purchased in Kaohsiung, Taiwan. In water samples, Sb(V) was the major species where as in juices organometallic Sb species were also present.
开发了一种通过离子色谱（IC）与电感耦合等离子体质谱（ICP-MS）联用的方法来进行锑的形态分析。在本研究中，使用以Hamilton PRP-X100柱为固定相的阴离子交换色谱法，在不到8分钟的时间内分离了亚锑酸盐[Sb(III)]、锑酸盐[Sb(V)]和三甲基锑(V)（TMeSb）等锑形态。流动相A为20mmol/L乙二胺四乙酸（EDTA）、2mmol/L邻苯二甲酸氢钾（KHP）于1% v/v甲醇中（pH 5.5），20mmol/L EDTA、2mmol/L KHP、40mmol/L(NH₄)₂CO₃于1% v/v甲醇中（pH 9.0）形成流动相B。检测限和相对标准偏差（RSD）分别为0.012 - 0.032ng/mL和2.2 - 2.8%。该方法应用于在台湾高雄购买的瓶装水和果汁。在水样中主要形态为Sb(V)，而在果汁中也存在有机金属锑形态。
