Kuzin M I, Kharnas S S, Zavodnov V Ia, Okhotnikova N L, Loshchenov V B, Volkova A I, Beliaeva L A
Khirurgiia (Mosk). 2000(11):11-4.
The results of examination of 47 patients with confirmed diagnosis of cancer of the stomach (21 patients) or 26 suspects were analyzed. 2-4 hours after oral taking of Alasence in the dose 5-10 mg/kg, gastroscopy with spectrofluorescence analysis of ALA-induced protoporphyrin IX was performed. There were no toxic complications due to Alasence taking. The standard spectrodetector LESA-7 was used for fluorescence record registration. The fluorescence spectra were recorded in the range of 650-850 nm. The specific for protoporphyrin IX peak at 700 nm wavelength was analyzed. Fluorescent endoscopic diagnosis allowed to confirm stomach cancer in 21 patients, and to detect cancer in 7 patients, including 2 cases of cancer in situ. The contrast of protoporphyrin IX accumulation (tumor/norm ratio) ranged from 2.5 to 18, that was enough for differential diagnosis in the majority of cases. In analysis of spectrograms for correct contrast calculation it is necessary to take into account the own fluorescence, with was high in some cases.
对47例确诊为胃癌的患者（21例）或26例疑似患者的检查结果进行了分析。口服剂量为5 - 10 mg/kg的阿拉森（Alasence）2 - 4小时后，进行胃镜检查并对ALA诱导的原卟啉IX进行光谱荧光分析。服用阿拉森未出现毒性并发症。使用标准光谱探测器LESA - 7记录荧光。荧光光谱记录范围为650 - 850 nm。分析了波长700 nm处原卟啉IX的特异性峰。荧光内镜诊断在21例患者中确诊为胃癌，在7例患者中检测到癌症，包括2例原位癌。原卟啉IX积累的对比度（肿瘤/正常组织比值）在2.5至18之间，这在大多数情况下足以进行鉴别诊断。在分析用于正确对比度计算的光谱图时，有必要考虑自身荧光，在某些情况下自身荧光较高。
