Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Institute of Photomedicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, China.
Photodiagnosis Photodyn Ther. 2017 Sep;19:304-307. doi: 10.1016/j.pdpdt.2017.05.016. Epub 2017 Jun 15.
In psoriatic lesions, fluorescence diagnosis with blue light can detect protoporphyrin IX accumulation, especially after topical 5-aminolaevulinic acid (ALA) application. However, variable fluorescence distributions, interpersonal variations and long incubation time limit its wide application in clinic. This study is aimed to identify a consistent and convenient method to facilitate diagnosis and evaluation of psoriatic lesions.
104 psoriatic lesions from 30 patients were evaluated. Single lesion PSI scoring and fluorescence by macrospectrofluorometry were recorded on each lesion before and after treatment with narrow-band UVB.
Punctate red fluorescence, emitted mainly by protoporphyrin IX, is observed in some psoriatic lesions. According to psoriasis severity index, fluorescence-positive lesions are more severe than lesions without fluorescence. We found a significant positive correlation between psoriasis severity and fluorescence intensity from protoporphyrin IX.
Protoporphyrin IX-induced red fluorescence can be used as a novel and convenient approach for psoriasis diagnosis and progression evaluation.
在银屑病皮损中，使用蓝光进行荧光诊断可以检测到原卟啉 IX 的积累，尤其是在局部应用 5-氨基酮戊酸（ALA）后。然而，荧光分布的可变性、个体间的差异和较长的孵育时间限制了其在临床上的广泛应用。本研究旨在寻找一种一致且方便的方法，以促进银屑病皮损的诊断和评估。
对 30 例患者的 104 处银屑病皮损进行评估。在接受窄谱 UVB 治疗前后，对每个皮损进行单病灶 PSI 评分和宏观荧光光谱仪的荧光检测。
在一些银屑病皮损中观察到点状红色荧光，主要由原卟啉 IX 发出。根据银屑病严重程度指数，荧光阳性皮损比无荧光皮损更严重。我们发现银屑病严重程度与原卟啉 IX 荧光强度之间存在显著的正相关关系。
原卟啉 IX 诱导的红色荧光可作为一种新的、方便的银屑病诊断和进展评估方法。
