Volchek N A, Gupalova T V, Totolian A A
Research Institute of Experimental Medicine, St. Petersburg, Russia.
Zh Mikrobiol Epidemiol Immunobiol. 2000 Sep-Oct(5):7-11.
The treatment of streptococci, groups C and G, with bromocyanogen made it possible to isolate surface G protein, capable of binding human serum albumin (HSA) and polyclonal IgG. In this work the presence of G protein in all staphylococcal strains, groups C and G, is shown. The differences between the strains by the level of expression, molecular weight and functional activity of G protein, extracted from streptococci of groups C and G, permitted the identification of 3 groups of strains, containing the molecules of G protein with different numbers of IgG- and HSA-binding domains: with 3 IgG- and HSA-binding domains, with 2 IgG- and HSA-binding domains and with only 2 IgG-binding domains. Each strain under study expressed only one of the molecule of G protein. The work shows the possibility of the identification of streptococci, groups C and G, by the molecular characteristics of G proteins themselves and their respective coding genes.
用溴化氰处理C组和G组链球菌，使得分离出能够结合人血清白蛋白（HSA）和多克隆IgG的表面G蛋白成为可能。在这项研究中，显示了所有C组和G组葡萄球菌菌株中均存在G蛋白。从C组和G组链球菌中提取的G蛋白，在表达水平、分子量和功能活性方面存在差异，这使得能够鉴定出3组菌株，它们所含G蛋白分子具有不同数量的IgG和HSA结合结构域：具有3个IgG和HSA结合结构域、具有2个IgG和HSA结合结构域以及仅具有2个IgG结合结构域。所研究的每个菌株仅表达一种G蛋白分子。这项研究表明，通过G蛋白本身及其各自编码基因的分子特征来鉴定C组和G组链球菌是有可能的。
