Li L, Wang A, He P, Fang Y
Department of Chemistry, East China Normal University, Shanghai.
Fresenius J Anal Chem. 2000 Aug;367(7):649-52. doi: 10.1007/s002160000424.
Thiocyanate traces have a strong inhibitory effect on the oxidation of Neutral Red by potassium bromate under UV irradiation in diluted phosphoric acid. Neutral Red exhibits a sensitive second derivative oscillopolarographic wave at -0.6 V(vs. SCE) in diluted phosphoric acid and sodium acetate solution. The oscillopolarographic behavior of Neutral Red was selected as indicator component for its photo-activated oxidation. The photochemical reaction rate equation was determined. A detection limit of 0.3 ng mL(-1) (3sigma/k) and a linear calibration curve from 2.0-48.0 ng mL(-1) thiocyanate were obtained. The method was applied to the determination of thiocyanate in urine, saliva and serum with satisfactory results.
在稀磷酸中，紫外光照射下，痕量硫氰酸盐对溴酸钾氧化中性红有强烈的抑制作用。在稀磷酸和醋酸钠溶液中，中性红在-0.6V（vs.SCE）处呈现灵敏的二阶导数示波极谱波。选择中性红的示波极谱行为作为其光活化氧化的指示组分，确定了光化学反应速率方程。得到硫氰酸盐的检测限为0.3 ng mL⁻¹（3σ/k），线性校准曲线范围为2.0 - 48.0 ng mL⁻¹。该方法应用于尿液、唾液和血清中硫氰酸盐的测定，结果令人满意。
